For research use only. We do not sell to patients.
Ionizable lipids / CKK-E12
CKK-E12 chemical structure
Product imageResearch use only
CAT. NO. DC49882Featured

CKK-E12

Pack size & price

USD
TotalUS$450
We match the best price and quality on market.
Ships from ShanghaiIce-pack transportPure lipid recommended
Product overview

Description & Application

cKK-E12 is an ionizable lipopeptide lipid investigated for hepatocyte-selective siRNA delivery. Published studies report potent factor VII silencing in rodents and greater than 95% liver TTR mRNA silencing in cynomolgus monkeys at 0.3 mg/kg siRNA.

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Literature evidence · DC49882

Formulation & Performance

The 2014 PNAS paper provides mouse, rat and nonhuman-primate siRNA evidence. Rodent ED50 values, cell-selectivity experiments and the monkey fixed-dose TTR result are shown separately.

DC49882 is cKK-E12. The catalog molecular weight 993.62 is retained. This module reports the original siRNA study; results from later mRNA formulations are not merged into its formulation record.

Formulation components and molecular-weight conversion

ComponentDC Chemicals productMolecular weightMolar ratioMass at 1 µmol total lipid (mg)
cKK-E12DC49882993.62Not assignedNot calculable from disclosed data
DSPCDC57005790.15Not assignedNot calculable from disclosed data
CholesterolDCD-041386.65Not assignedNot calculable from disclosed data
PEG-lipid (identity unassigned)Not uniquely mappedNot establishedNot assignedNot calculable from disclosed data

Main text identifies cKK-E12, DSPC, cholesterol and a PEG-lipid. The complete 2014 SI was not retrieved, so molar ratios, PEG identity/MW, N/P and lipid:RNA mass ratio are not assigned. A familiar 50:10:38.5:1.5 recipe is not assumed to be the verified original formulation.

Component mass calculator

Not all inputs are assigned to a single verified study formulation; unassigned fields are blank. Enter your own values to calculate a research scenario. Molecular weights and ratios are editable. Results are calculated, not measured study data. No N/P or RNA dose is inferred.

ComponentMWMolar ratioMass (mg)Mass %
cKK-E12——
DSPC——
Cholesterol——
PEG-lipid (identity unassigned)——

Formulation and physical characterization

Study / parameterReported evidenceSource / interpretation
Reported component classescKK-E12 / DSPC / cholesterol / PEG-lipid with siRNAMain Results; complete proportions remain unverified
DLS diameterApproximately 70 nmMain text and Fig. 2; approximate study value
Cryo-TEMParticles approximately 35–85 nmMain Fig. 2; imaging and hydrodynamic size are different measurements
Unassigned parametersExact PEG identity, proportions, N/P, encapsulation percentage and PDIComplete original SI required before these values are prefilled

Rodent siRNA potency

Study / parameterReported evidenceSource / interpretation
Mouse FVIIED50 approximately 0.002 mg/kg entrapped siRNAMain Fig. 2; a factor VII silencing endpoint
Rat FVIIED50 below 0.01 mg/kg siRNAMain Results; species-specific potency estimate
Dose interpretationValues are siRNA dose-based, not lipid dose-basedNot an mRNA expression dose or reporter-editing percentage
Benchmark interpretationThe paper compares dose-response profiles of multiple delivery systemsDo not rank every application by one FVII assay

Cell selectivity and uptake mechanism

Study / parameterReported evidenceSource / interpretation
Pten silencing0.1 mg/kg siRNA produced >80% silencing in mouse hepatocytesMain Fig. 4; cell-resolved Pten experiment
Other liver populationsNo significant Pten silencing in endothelial cells or leukocytes at that doseSame Pten experiment; not an absolute ban on extrahepatic delivery
Separate target assaysTie2 and CD45 studies used 1 mg/kg and gave weaker cell-population responsesMain Fig. 4; different genes/doses cannot be treated as a single selectivity ratio
Apolipoprotein EApoE enhanced uptake/potency; activity fell in ApoE-deficient mice but remained in LDLR-deficient miceMain Fig. 5; supports an ApoE-associated mechanism without requiring LDLR alone

Nonhuman-primate TTR silencing

Study / parameterReported evidenceSource / interpretation
Species and cohortCynomolgus monkeys; n = 3Main Fig. 6
TreatmentSingle intravenous infusion of 0.3 mg/kg siTTR-containing LPNs15-minute infusion in the reported study
MeasurementLiver biopsy at 48 h; TTR mRNA measured relative to GAPDHMain Fig. 6; liver transcript endpoint
OutcomeGreater than 95% TTR mRNA silencing0.3 mg/kg is a tested effective dose, not a reported primate ED50
ScopeNo clinical response or long-term primate safety conclusion is established by this resultDo not equate liver mRNA silencing with serum protein reduction

Tolerability and remaining evidence gaps

Study / parameterReported evidenceSource / interpretation
Rat tolerabilityPaper reports tolerability at 1 mg/kg siRNASupporting toxicology Table S2 was not independently retrieved
Safety margin descriptionPaper describes a dose above 100 times the rat FVII ED50Study-specific comparison; not a validated human therapeutic index
Published correctionThe 2014 correction adds funding acknowledgmentsDOI 10.1073/pnas.1404711111; no efficacy-data correction described
Remaining gapsComplete original SI and exact formulation mappingNo invented NOAEL, immune-response panel or recipe

Original study figures

Original Figs. 2, 4–6: particle properties, cell selectivity, ApoE and monkey TTR response — Original source figures.

References and source locations

  1. Dong Y, Love KT, Dorkin JR et al. Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates. PNAS 111, 3955–3960 (2014). DOI: 10.1073/pnas.1322937111.
  2. Correction: additional funding acknowledgments. PNAS 111, 5753 (2014). DOI: 10.1073/pnas.1404711111.
CAS No.1432494-65-9
Chemical NameCKK-E12
Synonyms2,5-Piperazinedione, 3,6-bis[4-[bis(2-hydroxydodecyl)amino]butyl]-;CKKE12， CKK E12
SMILESN1C(CCCCN(CC(O)CCCCCCCCCC)CC(O)CCCCCCCCCC)C(=O)NC(CCCCN(CC(O)CCCCCCCCCC)CC(O)CCCCCCCCCC)C1=O
FormulaC60H120N4O6
M.Wt993.62
Purity>95% by ELSD-HPLC
StoragePure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
Shipping conditionShips from Shanghai. Pure lipid is stable during ice-pack transport.
Publication
  1. Dong Y, Love KT, Dorkin JR et al. Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates. PNAS 111, 3955–3960 (2014). DOI: 10.1073/pnas.1322937111.
  2. Correction: additional funding acknowledgments. PNAS 111, 5753 (2014). DOI: 10.1073/pnas.1404711111.
F&PFormulation & PerformanceComposition, study results and referencesRead online →
Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

Mass = concentration × volume × molecular weight
Enter any three values to calculate the fourth.
C₁V₁ = C₂V₂
Enter any three values to calculate the fourth.
Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass1 mM5 mM10 mM
10 mg10.0642 mL2.0128 mL1.0064 mL
25 mg25.1605 mL5.0321 mL2.5161 mL
50 mg50.3210 mL10.0642 mL5.0321 mL
100 mg100.6421 mL20.1284 mL10.0642 mL
250 mg251.6052 mL50.3210 mL25.1605 mL

Need a different pack size or technical document?

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Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?
We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.
Pure lipid or solution — which should I choose?
Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.
How should I measure a small amount of oily or viscous lipid?
Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.
Can you support formulation optimization and scale-up?
Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.