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CAT. NO. DC60510Featured

Iso-A11B5C1

Pack size & price

USD

TotalUS$1,100

We match the best price and quality on market.

Ships from ShanghaiIce-pack transportPure lipid recommended

Product overview

Description & Application

Iso-A11B5C1 is an ionizable lipid investigated for muscle-selective mRNA delivery after intramuscular administration. In the cited mouse study, its LNP formulation retained local expression while limiting detectable liver and spleen reporter expression relative to the SM-102 comparator.

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Literature evidence · DC60510

Formulation & Performance

The optimized muscle-delivery formulation uses a C14 PEG phospholipid. Its results should not be transferred to a formulation in which that component is replaced by DMG-PEG2000.

The results below describe the cited research formulations. Product specifications and commercial pack options are shown in the Specifications and ordering sections.

Formulation components and molecular-weight conversion

Component	DC Chemicals product	Molecular weight	Molar ratio	Mass at 1 µmol total lipid (mg)
Iso-A11B5C1	DC60510	662.1	60	0.397260
DOPE	DC33634	744.03	10	0.074403
Cholesterol	DCD-041	386.65	29	0.112128
C14-PEG2000 (14:0 PEG2000 PE)	Not uniquely mapped	Not established	1	Not calculable from disclosed data

Optimized molar composition = 60:10:29:1; ionizable lipid:mRNA = 10:1 by weight. The SI identifies 14:0 PEG2000 PE as the PEG lipid. Its exact commercial product mapping and molecular weight are not assigned here. This is not DMG-PEG2000; total mass fractions remain uncalculated until its molecular weight is specified. N/P was not reported.

Component mass calculator

Not all inputs are assigned to a single verified study formulation; unassigned fields are blank. Enter your own values to calculate a research scenario. Molecular weights and ratios are editable. Results are calculated, not measured study data. No N/P or RNA dose is inferred.

Total lipid (µmol)

Component	Mass (mg)	Mass %
Iso-A11B5C1	—	—
DOPE	—	—
Cholesterol	—	—
C14-PEG2000 (14:0 PEG2000 PE)	—	—

Formulation characteristics

Parameter	Evidence	Source / boundary
Apparent pKa	Approximately 7.2	Main Fig. 3B; formulation ionization measurement.
Size / PDI	Particle-size and dispersity measurements are plotted	SI Fig. S4, p. 9. No exact mean is transcribed from the graph.
Encapsulation	>80% was an optimization selection criterion	The criterion is not a final-batch numerical encapsulation result. SI Table S2 compares screening formulations.
Helper-lipid identity	DOPE; C14-PEG2000 phospholipid	Methods and SI. Unverified substitutions are not used in the component calculator.

Cell-line selectivity

Cell model	Comparison with SM-102 LNPs	Interpretation
HeLa	42-fold higher reporter expression	Main Fig. 3A; cell-line-specific comparison.
HepG2	28-fold lower reporter expression	Main Fig. 3A; supports reduced expression in this hepatic cell model.
Splenocytes	24-fold lower reporter expression	Main Fig. 3A; does not imply absence of particle exposure.

Intramuscular delivery and off-target expression

Study	Conditions	Reported outcome
Luciferase mRNA	C57BL/6J mice; 0.3 mg/kg IM; 6 h; n = 3	Local expression comparable to SM-102; no significant difference reported. Liver/spleen off-target expression was not detected under the assay conditions (Fig. 2).
Cre mRNA reporter	mTmG mice; 0.5 mg/kg IM; 36 h; n = 3	Muscle reporter activation comparable to SM-102; Iso-A11B5C1 activation not detected in liver/spleen (Fig. 4).
SM-102 Cre comparator	Same reporter study	83.83% liver and 72.67% spleen reporter activation reported for the comparator. These are reporter readouts, not injected-dose recovery.

Five tissue sections were averaged per mouse in the Cre analysis. Cre-dependent recombination is distinct from CRISPR editing. Undetected expression does not demonstrate zero tissue exposure.

Tumour response and acute tolerability

Endpoint	Reported evidence	Limit
Trp2 mRNA / B16F10 model	Doses administered on days 7 and 12; tumour control exceeded MC3 but was less effective than SM-102	Main Fig. 5; do not describe Iso-A11B5C1 as superior across all efficacy endpoints.
Acute cytokines	BALB/c mice, 0.5 mg/kg IM; six cytokines at 4 h showed no discernible difference from PBS	SI Fig. S8; one acute time point does not establish systemic or repeat-dose safety.

Original study figures

Original Figures 2–5 and Supporting Information — Original source figures.

References and source locations

CAS No.	Not available
Chemical Name	Iso-A11B5C1
Synonyms	IsoA11B5C1, Iso A11B5C1, iso-A11B5C1, H3
SMILES	CCCCCCCC/C=C\CCCCCCCCNC(=O)C(CCCCCOC(=O)CCC(C)CCCCC)NCCN1CCCC1
Formula	C41H79N3O3
M.Wt	662.10
Purity	>95% by ELSD-HPLC
Storage	Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
Shipping condition	Ships from Shanghai. Pure lipid is stable during ice-pack transport.
Publication	Chen J, Xu Y, Zhou M, et al. Combinatorial design of ionizable lipid nanoparticles for muscle-selective mRNA delivery with minimized off-target effects. PNAS 120, e2309472120 (2023). DOI: 10.1073/pnas.2309472120. Full text and Supporting Information, Table S2 and Figures S4/S8.

Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

Mass = concentration × volume × molecular weight

MassConcentrationVolumeMolecular weightg/mol

Enter any three values to calculate the fourth.

C₁V₁ = C₂V₂

C₁mMV₁mLC₂mMV₂mL

Enter any three values to calculate the fourth.

Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass	1 mM	5 mM	10 mM
10 mg	15.1035 mL	3.0207 mL	1.5103 mL
25 mg	37.7586 mL	7.5517 mL	3.7759 mL
50 mg	75.5173 mL	15.1035 mL	7.5517 mL
100 mg	151.0346 mL	30.2069 mL	15.1035 mL
250 mg	377.5865 mL	75.5173 mL	37.7586 mL

Need a different pack size or technical document?

Contact our team with the catalog number and requested quantity.

Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?

We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.

Pure lipid or solution — which should I choose?

Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.

How should I measure a small amount of oily or viscous lipid?

Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.

Can you support formulation optimization and scale-up?

Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.

Iso-A11B5C1

Pack size & price

Description & Application

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