Lipid TOT-5
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Ionizable lipid for LNP and nucleic-acid delivery research.
C18 NC-TNP is a novel noncationic thiourea lipid without positively charged groups. It binds nucleic acids via hydrogen bonds instead of electrostatic attraction, avoiding cation-triggered systemic inflammation. Formulated into nanoparticles, it efficiently encapsulates mRNA, siRNA and plasmids, shows excellent serum tolerance and long-term liquid/lyophilized storage stability. It enters cells mainly through macropinocytosis, escapes endosomes intact to reduce nucleic acid degradation. In vivo, it targets spleen preferentially, induces robust long-lasting Th1-type cellular and humoral immunity with minimal organ toxicity, superior to SM102 LNPs for mRNA cancer vaccine delivery.
| CAS No. | Not available |
| Chemical Name | C18 NC-TNP |
| Synonyms | C18 NC TNP , C18NCTNP |
| SMILES | CCCCCCCCCCCCCCCCCCOC(=O)CCC(C(=O)OCCCCCCCCCCCCCCCCCC)NC(NCC(O)CO)=S |
| Formula | C45H88O6N2S |
| M.Wt | 784.64 |
| Purity | 98% |
| Storage | Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light. |
| Shipping condition | Ships from Shanghai. Pure lipid is stable during ice-pack transport. |
| Publication | Biomimetic noncationic lipid nanoparticles for mRNA delivery (Wang et al.)-Proc Natl Acad Sci U S A . 2023 Dec 19;120(51) |
The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.
Solvent volumes update from the molecular weight above.
| Mass | 1 mM | 5 mM | 10 mM |
|---|---|---|---|
| 10 mg | 12.7447 mL | 2.5489 mL | 1.2745 mL |
| 25 mg | 31.8617 mL | 6.3723 mL | 3.1862 mL |
| 50 mg | 63.7235 mL | 12.7447 mL | 6.3723 mL |
| 100 mg | 127.4470 mL | 25.4894 mL | 12.7447 mL |
| 250 mg | 318.6175 mL | 63.7235 mL | 31.8617 mL |
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