For research use only. We do not sell to patients.
Ionizable lipids / C18 NC-TNP
C18 NC-TNP chemical structure
Product imageResearch use only
CAT. NO. DC60537Featured

C18 NC-TNP

Pack size & price

USD
Supporting components
Cholesterol DCD-041 · 100 mg

Components are supplied separately; this is not a premixed LNP. RNA/cargo, buffer and formulation service are not included.

Total$700
We match the best price and quality on market.
Ships from ShanghaiIce-pack transportPure lipid recommended
Product overview

Description & Application

C18 NC-TNP is a noncationic thiourea lipid studied in a two-component mRNA carrier with cholesterol. Published mouse experiments report prolonged expression after subcutaneous injection, lymph-node immune-cell delivery and spleen-associated distribution after intravenous injection.

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Literature evidence · DC60537

Formulation & Performance

C18 NC-TNP uses a noncationic delivery concept. Subcutaneous expression, intravenous distribution and immune responses are reported as distinct endpoints.

Reported performance refers to the complete research formulation and the stated experimental model.

Formulation components and molecular-weight conversion

ComponentDC Chemicals productMolecular weightMolar ratioMass at 1 µmol total lipid (mg)
C18 NC-TNPDC60537785.274600.471164
CholesterolDCD-041386.65400.154660

Noncationic thiourea lipid/cholesterol = 60:40 mol%. Source: main Methods, preparation of NC-TNP. The carrier does not use the four-component SM-102 comparator composition. N/P is not assigned to this hydrogen-bond-based noncationic formulation.

Component mass calculator

Prefilled proportions follow the source specified above. Molecular weights and ratios are editable. Results are calculated, not measured study data. No N/P or RNA dose is inferred.

ComponentMWMolar ratioMass (mg)Mass %
C18 NC-TNP——
Cholesterol——

Formulation and particle properties

Experiment / parameterStudy conditionsResult / interpretation
CompositionTwo-component carrierC18 thiourea lipid and cholesterol; the cited formulation contains no DSPC or PEG lipid.
Particle dimensionsMain Fig. 1CApproximately 100 nm; exact PDI and final encapsulation percentage are not inferred from plots.
Study comparatorSM-102/DSPC/cholesterol/DMG-PEG200050:10:38.5:1.5 mol%. This comparator is not the composition of C18 NC-TNP.

Cellular expression

Experiment / parameterStudy conditionsResult / interpretation
Reporter deliveryDC2.4, B16 and 4T1 cells; EGFP mRNA; 1 µg/mL, 48 hHigher reporter expression than the study LNP comparator was reported (main Fig. 1 and supplementary experiments).
InterpretationTested cell lines and conditionsThe study supports a full-formulation comparison, not guaranteed superiority in all cell types or with every RNA.

Subcutaneous expression and lymph-node delivery

Experiment / parameterStudy conditionsResult / interpretation
Luciferase durationC57BL/6 mice; 0.5 mg/kg mRNA SC; Fig. 4A,BSignal remained detectable at day 20; the comparator signal persisted for up to approximately 3 days.
Expression kineticsSame SC studyThe authors report approximately tenfold longer translation half-life. This is not a pharmacokinetic half-life for the isolated lipid.
Lymph-node functional deliveryAi9 mice; 0.1 mg/kg Cre mRNA SC footpad; 48 h; Fig. 4CMore than 14% of dendritic cells were reporter-positive, approximately twice the comparator. Other immune-cell populations were evaluated separately.

Intravenous distribution and immune response

Experiment / parameterStudy conditionsResult / interpretation
Organ distributionCy5-labelled carriers; 0.5 mg/kg mRNA IV; Fig. 4D–FGreater spleen-associated signal than the comparator. Label distribution is not equivalent to productive mRNA expression.
Antigen-specific responseOVA mRNA SC; 1 mg/kg; day 14 assessment; Fig. 5Enhanced dendritic-cell activation and antigen-specific T-cell responses were reported.
Memory responseOVA schedule days 0, 7 and 14; assessment day 60Supplementary experiments report memory and recall endpoints. These are antigen-model mouse findings, not clinical protection data.

Inflammation and formulation storage

Experiment / parameterStudy conditionsResult / interpretation
Route-specific inflammation20 µg/mouse; intradermal, intramuscular or intravenous; day 3The carrier produced lower inflammatory responses than the study LNP comparator under the tested conditions (Figs. 2–3).
Storage experimentLiquid and lyophilized formulations at 4°C for 30 days; Fig. 1E–GRelative reporter expression and particle changes were evaluated; activity retention was better than the comparator.
ScopeResearch formulationLower measured inflammation does not mean zero toxicity. Storage observations are not the shelf-life specification of the supplied neat lipid.

Original study figures

Cell expression, inflammation, SC expression and IV distribution: Figures 1–5 — Original source figures.

References and source locations

  1. Wang C, et al. Biomimetic noncationic lipid nanoparticles for mRNA delivery. PNAS 120, e2311276120 (2023). DOI: 10.1073/pnas.2311276120.
  2. Full text and SI Appendix: main Figs. 1–5; supplementary immune-memory experiments.
CAS No.Not available
Chemical NameC18 NC-TNP
SynonymsC18 NC TNP , C18NCTNP
SMILESCCCCCCCCCCCCCCCCCCOC(=O)CCC(C(=O)OCCCCCCCCCCCCCCCCCC)NC(NCC(O)CO)=S
FormulaC45H88O6N2S
M.Wt785.274
Purity>95% by ELSD-HPLC
StoragePure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
Shipping conditionShips from Shanghai. Pure lipid is stable during ice-pack transport.
Publication
  1. Wang C, et al. Biomimetic noncationic lipid nanoparticles for mRNA delivery. PNAS 120, e2311276120 (2023). DOI: 10.1073/pnas.2311276120.
  2. Full text and SI Appendix: main Figs. 1–5; supplementary immune-memory experiments.
Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

Mass = concentration × volume × molecular weight
Enter any three values to calculate the fourth.
C₁V₁ = C₂V₂
Enter any three values to calculate the fourth.
Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass1 mM5 mM10 mM
10 mg12.7344 mL2.5469 mL1.2734 mL
25 mg31.8360 mL6.3672 mL3.1836 mL
50 mg63.6720 mL12.7344 mL6.3672 mL
100 mg127.3441 mL25.4688 mL12.7344 mL
250 mg318.3602 mL63.6720 mL31.8360 mL

Need a different pack size or technical document?

Contact our team with the catalog number and requested quantity.

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Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?
We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.
Pure lipid or solution — which should I choose?
Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.
How should I measure a small amount of oily or viscous lipid?
Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.
Can you support formulation optimization and scale-up?
Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.