Product imageResearch use only

CAT. NO. DC60856Featured

DMA4-H228

Research highlights

Branched lipid tailsMouse spleen reporter deliveryAntigen mRNA research

Pack size & price

USD

Add the cited formulation componentsSelected DMA4-H228 pack + every supporting component at 100 mg each+US$450 / set

Supporting components

Cholesterol DCD-041 · 100 mg+DSPC DC57005 · 100 mg+DMG-PEG2000 DC40169 · 100 mg

Components are supplied separately; this is not a premixed LNP. RNA/cargo, buffer and formulation service are not included.

Total$550

We match the best price and quality on market.

Ships from ShanghaiIce-pack transportPure lipid recommended

Product overview

Description & Application

DMA4-H228 is a novel, biodegradable lipidoid specifically engineered for spleen-targeted mRNA delivery. Its structure combines a dimethylamino (DMA4) headgroup with a unique hyperbranched lipid tail (H228) incorporating ester bonds for enhanced biodegradability. This design enables the formation of stable lipid nanoparticles (LNPs) (~170 nm) with high mRNA encapsulation efficiency (>96%). Critically, DMA4-H228 exhibits exceptional intrinsic tropism for the spleen (>98% targeting efficiency after IV administration), requiring no external targeting ligands. It selectively delivers mRNA to splenic antigen-presenting cells (APCs), including dendritic cells, macrophages, and B cells. This triggers potent immune activation: rapid IFNα secretion, upregulation of APC maturation markers (CD86/CD40), and robust antigen-specific immune responses. Demonstrating significant therapeutic potential, DMA4-H228-based mRNA vaccines effectively inhibit tumor growth in melanoma models (e.g., B16F10-OVA). This correlates with increased tumor-infiltrating CD8⁺ T cells, a shift towards pro-inflammatory M1 macrophages, elevated antigen-specific antibodies (IgG), and strong T cell responses (evidenced by IFNγ⁺ spots). Its ability to bypass liver tropism and directly activate splenic APCs makes DMA4-H228 a powerful platform for next-generation mRNA vaccines and cancer immunotherapy.

CAS No.	Not available
Chemical Name	DMA4-H228
Synonyms	DMA4H228， DMA4 H228，
SMILES	CCCCCCCCN(CCCCCCCC)C(=O)CCC(=O)OCCOC(=O)CCN(CCC(=O)OCCOC(=O)CCC(=O)N(CCCCCCCC)CCCCCCCC)CCC1CCCN1C
Formula	C57H106N4O10
M.Wt	1007.49
Purity	>95% by ELSD-HPLC
Storage	Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
Shipping condition	Ships from Shanghai. Pure lipid is stable during ice-pack transport.
Publication	Dimethylamino-based synthetic lipidoid nanoparticles for selective mRNA delivery to splenic antigen-presenting cells. DOI: 10.1016/j.jconrel.2025.113737

Literature-reported formulation composition

Component	mol%	Role
DMA4-H228	66.67	Ionizable lipid
Cholesterol	25.67	Sterol
DSPC	6.67	Helper phospholipid
DMG-PEG2000	1	PEG lipid
Reported total	100.01	Source values retained

Component molecular weights and calculated amounts

Composition retained from the cited study below. Research planning aid only. Results cover lipid component weights and do not include RNA, buffer, solvent, process loss or an N/P ratio.

Total lipid mass (mg)

Component	Normalized mol%	Amount (µmol)	Weight (mg)
DMA4-H228	66.663	78.5537	79.1420
Cholesterol	25.667	30.2456	11.6944
DSPC	6.669	7.8589	6.2097
DMG-PEG2000	1.000	1.1782	2.9539

Calculated total: 100.0000 mg lipid.

Calculated catalog planning quantities; RNA, solvent and process loss are excluded. DMG-PEG2000 uses 2507. Component mass = total lipid mass × molar parts × molecular weight / sum(parts × molecular weight). This conversion does not infer N/P.

N/P ratioNot reported in the cited article

Ionizable lipid:RNA mass ratio10:1

Buffer & pH25 mM sodium acetate, pH 5.2.

CargoFLuc mRNA; separate OVA mRNA vaccine studies

Administration routeIntravenous tail vein

DoseFLuc 0.25 mg/kg in Fig.1; OVA 10 micrograms/mouse per dose in separate studies

Animal modelBALB/c reporter screening; C57BL/6 tumor studies

Formulation processDropwise addition of ethanolic lipid solution to acetate; dialyse blank LNPs against PBS; combine blank LNPs with RNA by pipetting.

Literature-reported performance for the cited formulation

Literature-reportedSplenic share of reporter signal97.9% in the representative Fig.1E readout0.25 mg/kg FLuc mRNA IV; screen n=2; not percent injected dose · 6 hMain PDF p.4, Fig.1D-E

Literature Data DisclaimerAll formulation parameters and performance values shown in this section are derived from the cited publication and are provided for reference only. These results were obtained using the specific materials, formulation process, cargo, dose, analytical method, model and administration route described in that study. Unless otherwise stated, the data were not generated or independently verified by DC Chemicals. DC Chemicals supplies the lipid compound only and does not guarantee that customers will reproduce the reported LNP properties or biological performance.

The administration route shown above was used in the cited study and is not a clinical-use instruction or recommendation by DC Chemicals.

Cited literature for this formulation and performance datasetDimethylamino-based synthetic lipidoid nanoparticles for selective mRNA delivery to splenic antigen-presenting cells. DOI: 10.1016/j.jconrel.2025.113737 DOI: 10.1016/j.jconrel.2025.113737
Main article supplied; SI not supplied. 10:1 is ionizable-lipid:RNA by weight, not total-lipid:RNA or N/P. Rounded component values total 100.01.

Primary article

Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

Mass = concentration × volume × molecular weight

MassConcentrationVolumeMolecular weightg/mol

Enter any three values to calculate the fourth.

C₁V₁ = C₂V₂

C₁mMV₁mLC₂mMV₂mL

Enter any three values to calculate the fourth.

Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass	1 mM	5 mM	10 mM
10 mg	9.9257 mL	1.9851 mL	992.57 μL
25 mg	24.8141 mL	4.9628 mL	2.4814 mL
50 mg	49.6283 mL	9.9257 mL	4.9628 mL
100 mg	99.2566 mL	19.8513 mL	9.9257 mL
250 mg	248.1414 mL	49.6283 mL	24.8141 mL

Need a different pack size or technical document?

Contact our team with the catalog number and requested quantity.

Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?

We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.

Pure lipid or solution — which should I choose?

Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.

How should I measure a small amount of oily or viscous lipid?

Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.

Can you support formulation optimization and scale-up?

Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.

DMA4-H228

Pack size & price

Description & Application

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