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CAT. NO. DC67605Featured

PyCB lipid

Pack size & price

USD

Add the cited formulation componentsSelected PyCB lipid pack + every supporting component at 100 mg each+US$450 / set

Supporting components

ALC-0315 DC42537 · 100 mg+DSPC DC57005 · 100 mg

Components are supplied separately; this is not a premixed LNP. RNA/cargo, buffer and formulation service are not included.

Total$850

We match the best price and quality on market.

Ships from ShanghaiIce-pack transportPure lipid recommended

Product overview

Description & Application

PyCB lipid is a zwitterionic component studied in PEG-free, three-component LNPs for splenic mRNA delivery. With ALC-0315 and DSPC, the published ThrCo formulation supported immune-cell expression, antigen-specific T-cell responses and antitumour activity in mouse models.

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Literature evidence · DC67605

Formulation & Performance

The optimized ThrCo formulation and its immune-cell, repeat-dose and tumour-model results are presented separately from the earlier screening mixtures.

Reported performance refers to the complete research formulation and the stated experimental model.

Formulation components and molecular-weight conversion

Component	DC Chemicals product	Molecular weight	Molar ratio	Mass at 1 µmol total lipid (mg)
ALC-0315	DC42537	766.27	35.6	0.272792
PyCB lipid	DC67605	929.42	44.4	0.412662
DSPC	DC57005	790.15	20	0.158030

ALC-0315/PyCB/DSPC = 35.6:44.4:20 mol%. Source: Supplementary Materials, optimized ThrCo LNP composition (p. 3). This formulation contains neither cholesterol nor PEG lipid. N/P and lipid:RNA mass ratio were not established; neither is inferred from the molar composition.

Component mass calculator

Prefilled proportions follow the source specified above. Molecular weights and ratios are editable. Results are calculated, not measured study data. No N/P or RNA dose is inferred.

Total lipid (µmol)

Component	Mass (mg)	Mass %
ALC-0315	—	—
PyCB lipid	—	—
DSPC	—	—

Formulation selection and particle measurements

Experiment / parameter	Study conditions	Result / interpretation
Optimized composition	Three-component ThrCo LNP	35.6:44.4:20 mol%; use this final composition rather than ambiguous labels in the earlier screen.
Size, PDI and loading	Main Fig. 2; Supplementary Fig. S3	Comparative particle and encapsulation data are plotted. Exact final values are not transcribed from graph heights.
Laboratory comparator	ALC-0315/DSPC/cholesterol/DMG-PEG2000, 50:10:38.5:1.5	The authors describe this as a BNT162b2 LNP comparator; it is a laboratory formulation and is not the licensed vaccine product.

Splenic expression and cell delivery

Experiment / parameter	Study conditions	Result / interpretation
Luciferase expression	IV mouse reporter experiments; main Fig. 2 and Fig. S6	Authors report 4.5-fold higher splenic signal than the laboratory ALC-0315 comparator and >40-fold higher signal than the 18PA formulation.
Benchmark imaging conditions	Supplementary Fig. S6	0.25 mg/kg luciferase mRNA; imaging 6 h after IV administration.
Functional reporter delivery	Cre mRNA in Ai14 reporter mice; main Fig. 3	Splenic dendritic cells and macrophages showed reporter activation. This is Cre-mediated recombination, not a CAR-T or Cas9 editing result.

Antigen response and repeat administration

Experiment / parameter	Study conditions	Result / interpretation
Antigen-specific CD8 T cells	OVA mRNA, 5 µg/mouse IV on days 0, 7 and 14; assessed day 17; n = 3	Tetramer-positive CD8 T cells: 9.06% for ThrCo, 3.96% for the laboratory comparator and 0.57% for PBS (Fig. 4D,E).
Repeated delivery	Two weekly EGFP-mRNA doses followed by luciferase mRNA; 5 µg/mouse; final imaging at 24 h	Splenic expression was better retained than with the PEG-containing comparator (Fig. 4F–I). This does not establish indefinite repeat-dose performance.

Tumour-model evidence

Experiment / parameter	Study conditions	Result / interpretation
B16F10-OVA lung metastasis model	15 µg OVA mRNA/mouse on days 1, 6 and 11; endpoint day 21	The ThrCo group had no detectable lung nodules at the reported endpoint (Fig. 5A–C).
Interpretation	Antigen-defined mouse tumour model	The result is specific to this vaccination schedule and model; it is not evidence of universal tumour eradication or human efficacy.

Tolerability and storage

Experiment / parameter	Study conditions	Result / interpretation
Liver chemistry	IV mRNA at 1 or 5 mg/kg; 24 h; Fig. 2N–Q	ALT/AST were evaluated; no significant elevation was reported at 1 mg/kg. The higher-dose comparison does not establish chronic safety.
Hemolysis	Main Fig. 3P,Q	The formulation showed low hemolysis under the tested physiological-pH conditions; this endpoint does not replace systemic toxicology.
Short-term storage	Supplementary Fig. S5D–G	Reporter activity was evaluated after 4°C for 48 h, −80°C for one week and 37°C for 24 h. These are formulation experiments, not the isolated lipid shelf life.

Original study figures

Splenic delivery, repeat dosing and tumour outcomes: Figures 2–5 — Original source figures.

References and source locations

CAS No.	Not available
Chemical Name	PyCB lipid
Synonyms	PyCBlipid, PyCB-lipid
SMILES	CCCCCCCCC(CCCCCC)C(=O)OCCCCCCN(CCCCCCOC(=O)C(CCCCCC)CCCCCCCC)CCCCOC(=O)C1=CC=[N+](CC(=O)[O-])C=C1
Formula	C56H100N2O8
M.Wt	929.42
Purity	>95% by ELSD-HPLC
Storage	Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
Shipping condition	Ships from Shanghai. Pure lipid is stable during ice-pack transport.
Publication	Zhao Y, et al. Replacing cholesterol and PEGylated lipids with zwitterionic ionizable lipids in LNPs for spleen-specific mRNA translation. Science Advances 11, eady6460 (2025). DOI: 10.1126/sciadv.ady6460. Full text and Supplementary Materials: composition p. 3; Figs. S3, S5 and S6; main Figs. 2–5.

Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

Mass = concentration × volume × molecular weight

MassConcentrationVolumeMolecular weightg/mol

Enter any three values to calculate the fourth.

C₁V₁ = C₂V₂

C₁mMV₁mLC₂mMV₂mL

Enter any three values to calculate the fourth.

Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass	1 mM	5 mM	10 mM
10 mg	10.7594 mL	2.1519 mL	1.0759 mL
25 mg	26.8985 mL	5.3797 mL	2.6898 mL
50 mg	53.7970 mL	10.7594 mL	5.3797 mL
100 mg	107.5940 mL	21.5188 mL	10.7594 mL
250 mg	268.9850 mL	53.7970 mL	26.8985 mL

Need a different pack size or technical document?

Contact our team with the catalog number and requested quantity.

Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?

We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.

Pure lipid or solution — which should I choose?

Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.

How should I measure a small amount of oily or viscous lipid?

Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.

Can you support formulation optimization and scale-up?

Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.

PyCB lipid

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