Ships from ShanghaiIce-pack transportPure lipid recommended
Product overview
Description & Application
76-O17Se is a selenium-containing lipidoid investigated for intracellular delivery of mRNA and genome-editing proteins. In CAR-T cell engineering studies, 76-O17Se-based lipid nanoparticles delivered N1-methylpseudouridine-modified anti-CD19 CAR mRNA into CD8+ T lymphocytes. The resulting CAR-T cells demonstrated cytotoxic activity against B-cell lymphoma cells in vitro, supporting its application in nonviral mRNA delivery and transient immune-cell engineering research.
Anti-CD19 CAR-eGFP mRNA delivery was evaluated using 76-O17Se and related lipidoids. The observations below are tied to the explicitly labeled panels in the study's supporting information.
Formulation context
Active lipidoid
76-O17Se
System
Unformulated lipidoid nanoparticles. The article describes 76-O17Se without added helper lipids; a conventional four-component molar recipe is not assigned here.
Cargo
Anti-CD19 CAR-eGFP mRNA
Model / delivery
Human primary CD8+ T cells; in vitro cell transfection
Comparators
76-O17S, 82-O17O and untreated cells (Figure S5A)
N/P / preparation
N/P ratio, full mixing procedure and buffer conditions are not established by the supplied supporting information.
Sources: article formulation description; supporting information, Figure S5A, p. S6. The complete preparation method should be checked in the main article before reproducing the experiment.
T-cell expression
Figure S5A shows the anti-CD19-eGFP fluorescence distribution in human primary CD8+ T cells after delivery using 76-O17Se, alongside related lipidoids and an untreated control.
Figure S5A, p. S6. Original panel extracted from the supplied supporting information. The panel does not tabulate a numerical transfection percentage or replicate-level statistics.
Particle properties before and after mRNA loading
For the bars labeled 76-O17Se, mRNA loading is associated with a larger effective diameter, a modest increase in PDI and a lower positive zeta potential.
Property
Before loading (mRNA−)
After loading (mRNA+)
Effective diameter
Approximately 240 nm
Approximately 520 nm
PDI
Approximately 0.29
Approximately 0.35
Zeta potential
Approximately +46 mV
Approximately +28 mV
Figure-read estimates: rounded visual readings from Figure S2D–F, p. S4. The source provides bar plots, not a numerical data table; these are not exact reported means, batch specifications or acceptance criteria.
Figure S2D–F, p. S4. Original panels extracted from the supplied supporting information; the first pair of bars in each plot is 76-O17Se.
How to interpret this study
The supporting T-cell dose study also shows declining cell survival as dose increases (Figures S5B–C and S6). Reporter-positive percentages must be interpreted together with the live-cell gate, rather than treated as the percentage of all starting cells successfully engineered.
Figures S5B–C and S6 do not independently name the lipidoid in each dose panel. Their numerical dose–response values are therefore not assigned to 76-O17Se here. Figure S5C is headed “48 hr”, although its caption repeats “24 hr”.
The macrophage formulation data explicitly labeled 9322-O16B in Figures S2A–C and S3–S4 are separate from the 76-O17Se observations. They do not substantiate a claim that 76-O17Se outperforms Lipofectamine 2000 or MC3 in primary macrophages.
Source and evidence limits
Ye Z, Chen J, Zhao X, Li Y, Harmon J, Huang C, Chen J, Xu Q. In Vitro Engineering Chimeric Antigen Receptor Macrophages and T Cells by Lipid Nanoparticle-Mediated mRNA Delivery. ACS Biomaterials Science & Engineering. 2022;8(2):722–733. DOI: 10.1021/acsbiomaterials.1c01532. View publication →
Supporting information: ab1c01532_si_001, 8 pages, supplied for this product update. Directly labeled evidence: S2D–F and S5A. Main-article bibliographic details were verified; the full main text was not accessible during this update. No numerical N/P, encapsulation efficiency, pKa or complete preparation protocol is inferred.
Values shown are literature-reported observations or explicitly labeled figure-read estimates for the cited study conditions. They do not guarantee performance of the supplied product in other formulations or experiments.
Laboratory planning
Solution Calculators
The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.
Mass = concentration × volume × molecular weight
Enter any three values to calculate the fourth.
C₁V₁ = C₂V₂
Enter any three values to calculate the fourth.
Preparing stock solutions
Quick preparation table
Solvent volumes update from the molecular weight above.
Mass
1 mM
5 mM
10 mM
10 mg
11.5594 mL
2.3119 mL
1.1559 mL
25 mg
28.8984 mL
5.7797 mL
2.8898 mL
50 mg
57.7968 mL
11.5594 mL
5.7797 mL
100 mg
115.5936 mL
23.1187 mL
11.5594 mL
250 mg
288.9839 mL
57.7968 mL
28.8984 mL
Need a different pack size or technical document?
Contact our team with the catalog number and requested quantity.
How is the lipid shipped, and how should I store it?
We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.
Pure lipid or solution — which should I choose?
Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.
How should I measure a small amount of oily or viscous lipid?
Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.
Can you support formulation optimization and scale-up?
Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.
