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Lipid TD5 chemical structure View larger
Verified 2D structureFor research use only. We do not sell to patients.
CAT. NO. DC67521
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Lipid TD5

Literature-reported LNP dataNot a product specification or performance guarantee.View source

Available sizes & price

USD · research grade
Supporting components
DOPE DC33634 · 100 mg+Cholesterol DCD-041 · 100 mg+DMG-PEG2000 DC40169 · 100 mg

Components are supplied separately; this is not a premixed LNP. RNA/cargo, buffer and formulation service are not included.

Selected · total price$700
We match the best price and quality on market.
Ships from ShanghaiIce-pack transportPure lipid recommended
Description & Application

Brain-targeted ionizable lipid for CNS mRNA research

TD5 is an ionizable lipid investigated for mRNA delivery to the central nervous system after intrathecal injection. Mouse studies report neuronal and astrocyte expression, Cre-reporter activation and delivery of a CRISPR cargo. These findings concern intrathecal administration rather than intravenous blood-brain-barrier crossing.

Formulation contextFour-component LNP reported
Defined structureC₄₅H₈₁N₃O · MW 680.16
CNS researchIntrathecal administration studied
RNA cargomRNA and gene-editing workflows
Core parameter · pKa

The TD5 pH switch

An apparent pKa of 7.30 was reported for the formulated LNP. The value is presented with its measurement context in Product data.

Bloodstream · pH 7.4

Near-neutral surface

Reduced nonspecific electrostatic interactions while the nanoparticle travels through biological fluid.

Endosome · acidic pH

Progressive protonation

Ionizable amines gain charge as pH falls, supporting interaction with endosomal membranes.

Cytosol

Cargo release

Membrane disruption and escape allow encapsulated RNA to reach the cytosol for expression.

Product data

Technical details and supporting files

Open full Formulation & Performance →

Literature evidence · DC67521

Formulation & Performance

Full-text brain expression and tolerability results are now separated by reporter system. The quantitative lipid composition remains unconfirmed pending accessible Supporting Information.

Reported performance refers to the complete research formulation and the stated experimental model.

Formulation components and molecular-weight conversion

ComponentDC Chemicals productMolecular weightMolar ratioMass at 1 µmol total lipid (mg)
TD5DC67521680.16Not assignedNot calculable from disclosed data
DOPEDC33634744.03Not assignedNot calculable from disclosed data
CholesterolDCD-041386.65Not assignedNot calculable from disclosed data
DMG-PEG2000DC401692507Not assignedNot calculable from disclosed data

The paper identifies TD5, DOPE, cholesterol and DMG-PEG2000. Complete preparation Methods are in the Supporting Information, which was not accessible for this review. Quantitative molar ratios, N/P and lipid:RNA mass ratio could not be verified from the accessible full manuscript and are left unassigned.

Component mass calculator

Not all inputs are assigned to a single verified study formulation; unassigned fields are blank. Enter your own values to calculate a research scenario. Molecular weights and ratios are editable. Results are calculated, not measured study data. No N/P or RNA dose is inferred.

ComponentMWMolar ratioMass (mg)Mass %
TD5——
DOPE——
Cholesterol——
DMG-PEG2000——

Particle properties and cellular delivery

Experiment / parameterStudy conditionsResult / interpretation
Particle size / encapsulationTD5 BLNP characterization; Fig. 2107.5 ± 5.3 nm and 81.7 ± 2.2% encapsulation, as reported in the text.
Apparent pKaMain text referring to Supplementary Fig. S37.30. Exact PDI and zeta-potential values are not transcribed from graphs.
SH-SY5Y expressionNeuroblastoma cells; Fig. 2Luciferase expression was 50.1-fold above the MC3 comparator. GFP-positive cells: 80.8 ± 1.4% versus 4.4 ± 0.7%.

Intrathecal brain reporter expression

Experiment / parameterStudy conditionsResult / interpretation
Luciferase experiment0.135 mg/kg mRNA intrathecally; Fig. 3Brain signal exceeded the MC3 comparator by >42-fold; brain expression was approximately tenfold spinal-cord expression.
Organ expression selectivitySame reporter experimentBrain expression exceeded liver and spleen by >300-fold. This is an expression comparison, not the fraction of injected particles reaching brain.
GFP-positive neurons / astrocytes0.135 mg/kg intrathecally; Fig. 329.6 ± 10.7% / 38.1 ± 6.7%; MC3: 6.2 ± 1.2% / 6.3 ± 1.9%.
GFP-positive microglia / brain endothelial cellsSame GFP study12.9 ± 2.2% / 26.9 ± 4.3%; MC3: 2.8 ± 0.9% / 8.8 ± 1.9%.

Percentages refer to each identified cell population. The GFP assessment time is not newly assigned without the full Supporting Information.

Cre-mediated functional delivery

Experiment / parameterStudy conditionsResult / interpretation
Ai14 reporter miceCre mRNA, 0.135 mg/kg intrathecally; Fig. 4tdTomato-positive neurons: hippocampus 34.4 ± 4.0%, thalamus 29.5 ± 3.1%, cortex 31.4 ± 4.1%.
Astrocyte reporter activationSame Cre experimentHippocampus 44.8 ± 3.9%, thalamus 33.6 ± 6.6%, cortex 41.0 ± 4.8%.
Endpoint distinctionCre reporter activationThese percentages measure Cre-mediated recombination and must not be presented as Cas9 editing efficiencies.

CRISPR-cargo evidence

Experiment / parameterStudy conditionsResult / interpretation
Brain reporter activationCas9 mRNA/sgRNA; 0.135 mg/kg total RNA intrathecally; day 5; Fig. 5The study reports brain tdTomato activation with limited liver/spleen signal.
Quantitative boundaryImaging and tissue reporter evidenceNo numerical genomic editing efficiency or comprehensive off-target assessment is assigned from these images.

Tolerability and route limitations

Experiment / parameterStudy conditionsResult / interpretation
Short-term tolerability0.135 mg/kg intrathecally; 6 and 24 h; Fig. 6Cytokine responses were comparable to or weaker than MC3 for the reported markers, with most returning toward baseline at 24 h.
Chemistry and histologyMain Fig. 6 and supplementary results cited in textALT/AST, BUN and tissue observations were reported as favourable under the tested conditions.
Route specificityIntrathecal administrationThese experiments do not demonstrate IV delivery across an intact BBB, long-term neurotoxicity clearance or human safety.

Original study figures

Intrathecal reporter delivery, Cre and CRISPR evidence, and tolerability: Figures 3–6 — Original source figures.

References and source locations

  1. Xue Y, Wang C, Li H, et al. Lipid Nanoparticles Enhance mRNA Delivery to the Central Nervous System Upon Intrathecal Injection. Advanced Materials 37, 2417097 (2025). DOI: 10.1002/adma.202417097.
  2. Full author manuscript: Figures 2–6.

Specifications

PropertyValue
CAS No.2855180-86-6
Chemical NameLipid TD5
SynonymsLipid-TD5; TD5; TD-5
SMILESCCCCCCCCCCCCCCN(CCCCCCCCCCCCCC)CCCCCCNC(=O)CCC1=CNC2=CC=CC=C12
FormulaC45H81N3O
M.Wt680.16
Purity>95% by ELSD-HPLC
StoragePure form: −20 °C, 1 year. In solvent: −80 °C, 6 months; −20 °C, 1 month. Keep sealed and protected from light.
Shipping conditionPure lipid, shipped from Shanghai with ice packs.
Publication
  1. Xue Y, Wang C, Li H, et al. Lipid Nanoparticles Enhance mRNA Delivery to the Central Nervous System Upon Intrathecal Injection. Advanced Materials 37, 2417097 (2025). DOI: 10.1002/adma.202417097.
  2. Full author manuscript: Figures 2–6.
Lab tools

Solution calculators

Molarity calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Molecular weight is prefilled from this product and can be edited.

Dilution calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final) · C₁V₁ = C₂V₂

Leave exactly one field blank. With the current values, V₁ will be calculated.
Preparing stock solutions

Quick preparation table

Enter the molecular weight above to generate solvent volumes. Formula: volume (mL) = mass (mg) × 1000 ÷ [molecular weight (g/mol) × concentration (mM)].

Mass1 mM5 mM10 mM
Ethanol is a common starting solvent; confirm product-specific solubility before preparation.
Handling guidance

Shipping, storage and sampling

We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.
Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.
Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.
Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.

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