Lipid F10T5
Recommended because it shares a closely related research category or delivery context. Formulation and performance are product- and study-specific.
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Ionizable lipid for LNP and nucleic-acid delivery research.
C14-306 is a rationally designed ionizable lipid for brain targeting delivery, characterized by a linear 3,3'-diamino-N-methyldipropylamine (306) core conjugated with tetradecyl (C14) tails. This specific architectural configuration, synthesized via epoxide ring-opening amination, yields a molecular structure that optimally balances hydrophobic character and protonation capacity. The C14 alkyl chains enhance membrane integration and LNP stability, while the multiamine core facilitates efficient mRNA complexation and pH-dependent endosomal disruption. When formulated into LNPs with standard helper lipids (DOPE, cholesterol, DMG-PEG2000), C14-306-based nanoparticles exhibit favorable physicochemical properties, including a monodisperse size distribution near 110 nm and high mRNA encapsulation efficiency (>84%). High-throughput in vivo barcoding screening identified C14-306 LNPs as lead candidates for brain delivery, demonstrating a significant tropism for neuronal cells over liver tissue. In validation studies, LNPs incorporating C14-306 achieved a 6.9-fold increase in luciferase mRNA transfection in the mouse brain compared to the SM-102 benchmark, coupled with a substantial reduction in hepatic off-target expression. Flow cytometry confirmed preferential transfection of NeuN+ neurons, and safety assessments indicated no significant blood-brain barrier compromise or induction of systemic inflammation. The efficacy of C14-306 is attributed to its tailored pKa, promoting extended circulation and enhanced endosomal escape within brain cells. C14-306 represents a promising platform for systemic mRNA therapeutics targeting neurological disorders.
| CAS No. | Not available |
| Chemical Name | C14-306 |
| Synonyms | C14306;C14 306 |
| SMILES | CCCCCCCCCCCCC(O)CN(CCCN(C)CCCN(CC(O)CCCCCCCCCCCC)CC(O)CCCCCCCCCCCC)CC(O)CCCCCCCCCCCC |
| Formula | C63H131O4N3 |
| M.Wt | 994.76 |
| Purity | ELSD-HPLC>95% |
| Storage | Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light. |
| Shipping condition | Ships from Shanghai. Pure lipid is stable during ice-pack transport. |
| Publication | Han EL, Kim D, Murray AM, Mrksich K, Hamilton AG, Tang S, Yoo S, Zhu AT, Tong ER, Palanki R, Hall ML, Bedingfield SK, Mitchell MJ. High-Throughput In Vivo Screening Identifies Structural Factors Driving mRNA Lipid Nanoparticle Delivery to the Brain. ACS Nano. 2026 Jan 19 |
The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.
Solvent volumes update from the molecular weight above.
| Mass | 1 mM | 5 mM | 10 mM |
|---|---|---|---|
| 10 mg | 10.0527 mL | 2.0105 mL | 1.0053 mL |
| 25 mg | 25.1317 mL | 5.0263 mL | 2.5132 mL |
| 50 mg | 50.2634 mL | 10.0527 mL | 5.0263 mL |
| 100 mg | 100.5268 mL | 20.1054 mL | 10.0527 mL |
| 250 mg | 251.3169 mL | 50.2634 mL | 25.1317 mL |
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