Product imageResearch use only

CAT. NO. DC67623Featured

MOCHOL GMP-like Quality

Research highlights

POPC/DOPE/CHEMS/MOCHOL = 6/24/23/47 mol%MOCHOL pKa 6.5; pH-controlled charge conversionRelease specification: >75% RNA encapsulation, PDI <0.200MOCHOL converts to cholesterol under thermal stress

Pack size & price

USD

Total$300

We match the best price and quality on market.

Ships from ShanghaiIce-pack transportPure lipid recommended

Product overview

Description & Application

MoChol is a cationic cholesterol derivative used as a membrane component in charge-reversible amphoteric nanoliposomes. It has been incorporated with CHEMS, DOPE and POPC into liposomal formulations for the delivery of anti-BCL-2 antisense oligonucleotides.

Manufacturing & quality capability

GMP-like quality support for advanced preclinical and clinical-stage programs

DC Chemicals can supply MOCHOL (DC67623) at a GMP-like quality level for customer toxicology programs and clinical-stage formulation development. Kg-scale production capability is available for qualified projects, subject to technical review, batch planning and quality agreement.

GMP-like quality level

Batch-specific identity, purity, residual-solvent, water, residue-on-ignition, endotoxin and bioburden testing are available. Quality documentation can be aligned with the customer's qualification package.

Toxicology and clinical-stage support

Suitable for use as a lipid raw material in customer-led toxicology studies and clinical-stage formulation development. Final suitability remains subject to the customer's formulation, QA and regulatory assessment.

Kg-scale manufacturing

Production can be planned from research quantities through kilogram scale, with project-specific lead time, batch size, analytical release testing and packaging arranged in advance.

Representative COA results — Batch DC6762306

99.8%Main peak purity

0.12%Unknown single impurity

0.19%Total impurities

<0.005 EU/mgEndotoxins

<10 CFU/gTAMC / TYMC

0.37 wt%Water content

COA issued for Batch DC6762306; manufacturing date 12 Aug 2026; retest/expiry date 12 Aug 2028. The complete batch COA is available in the Documentation section. Elemental-impurity results and the final conclusion are reported as TBD on this representative COA and should be confirmed in the release package for the supplied batch.

View batch COA

Quality and regulatory note: “GMP-like” describes the available manufacturing and quality-support level; it does not mean that this lipid itself is an approved drug substance, GMP-certified product, or regulatory-authorized clinical material. Customers remain responsible for qualification, formulation, sterility strategy, release criteria and regulatory use.

CAS No.	452323-21-6
Chemical Name	MOCHOL
Synonyms	Not available
SMILES	CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](OC(=O)CCC(=O)NCCN5CCOCC5)CC[C@]4(C)[C@H]3CC[C@@]21C
Formula	C37H62N2O4
M.Wt	598.47
Purity	>95% by ELSD-HPLC
Storage	Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
Shipping condition	Ships from Shanghai. Pure lipid is stable during ice-pack transport.
Publication	MiNA Therapeutics Ltd. C/EBP alpha saRNA compositions and methods of use. WO2016/170349 A1. Published 27 October 2016.

Literature-reported formulation composition

Component	mol%	Role
POPC	6	Helper phospholipid
DOPE	24	Helper phospholipid
CHEMS	23	anionic_sterol_lipid
MOCHOL	47	ionizable_sterol_lipid
Reported total	100	Source values retained

Formulation calculator unavailable. The 4 reported lipid components total 100 mol%. Obtain and verify the complete composition and component molecular weights before planning component quantities.

N/P ratioNot reported and not inferred.

Total lipid:cargo weight ratioOptimized by changing CEBPA-51 concentration while holding lipid concentration constant; no single total-lipid:RNA mass ratio is stated in the reviewed text.

Buffer & pHRNA phase: sodium acetate/sucrose pH 4.0. Immediate pH adjustment: NaCl/phosphate pH 9.0. Final exchange buffer: sucrose/phosphate pH 7.5.

CargoCEBPA-51 small activating RNA (saRNA)

Administration routeIntravenous infusion in the described product context; intravenous dosing in the rat cirrhosis example

Dose1 mg/kg MTL-CEBPA in the delayed-treatment rat cirrhosis example

Animal modelMale Sprague-Dawley rats with carbon-tetrachloride-induced cirrhosis

Formulation processCEBPA-51 in sodium acetate/sucrose pH 4.0 and lipids in absolute ethanol at 55 C were combined by crossflow ethanol injection. The mixture was immediately diluted with NaCl/phosphate pH 9.0, extruded through 0.2 micrometer polycarbonate membrane, ultrafiltered, diafiltered with at least seven volumes of sucrose/phosphate pH 7.5, and sterile filtered.

Literature-reported performance for the cited formulation

Literature-reportedProduct particle specification50-150 nm; PDI <0.200; zeta potential < -30 mV at pH 7.2-7.8MTL-CEBPA release specificationParagraph [000613]

Literature-reportedRNA encapsulation specification>75%RiboGreen total/free RNA methodParagraphs [000613]-[000615]

Literature-reportedProcess optimization yieldAbout 50-60%CEBPA-51 concentration and pH experimentsParagraphs [000794]-[000805], Figs. 34-35

Literature-reportedCirrhosis-model outcomesImproved body weight, liver-function markers, ammonia, total protein, ascites and survival1 mg/kg MTL-CEBPA started at week 8 or 11Paragraphs [000826]-[000830], Figs. 36-38

MOCHOL formulation and stability findings

Item	Condition	Reported result
Composition	NOV340 lipids	POPC/DOPE/CHEMS/MOCHOL = 6/24/23/47 mol%
Release profile	pH 7.2-7.8	>75% RNA encapsulation; 50-150 nm; PDI <0.200; zeta < -30 mV
Frozen stability	-20 +/- 5 C	No meaningful change reported through 6 months
Thermal stress	72 h at 25 C / 40 C	MOCHOL decreased 16% / 25% with cholesterol formation

WO2016/170349 A1 paragraphs [000597], [000613]-[000615], [000820]-[000825].

Additional reported findings

pH-dependent loading

MOCHOL is described as positively charged in the acidic RNA-loading phase (pKa 6.5); raising pH after vesicle formation produces the charge-converted final system.

NOV340/MTL-CEBPA process · Paragraphs [000599]-[000604], [000794]

Storage degradation pathway

Under 25-40 C stress, MOCHOL content fell while cholesterol increased; the other lipid excipients and total saRNA did not show significant changes in the described test.

Single stability batch · Paragraphs [000822]-[000824]

Literature Data DisclaimerAll formulation parameters and performance values shown in this section are derived from the cited publication and are provided for reference only. These results were obtained using the specific materials, formulation process, cargo, dose, analytical method, model and administration route described in that study. Unless otherwise stated, the data were not generated or independently verified by DC Chemicals. DC Chemicals supplies the lipid compound only and does not guarantee that customers will reproduce the reported LNP properties or biological performance.

The administration route shown above was used in the cited study and is not a clinical-use instruction or recommendation by DC Chemicals.

Cited literature for this formulation and performance datasetMiNA Therapeutics Ltd. C/EBP alpha saRNA compositions and methods of use. WO2016/170349 A1. Published 27 October 2016. DOI: https://patents.google.com/patent/WO2016170349A1/en
All formulation, stability and animal statements refer to the MOCHOL-containing NOV340/MTL-CEBPA system described in the patent.

WO2016/170349 A1

Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

Complete mass calculator disabled because POPC/CHEMS catalog mapping and CHEMS/MOCHOL molecular-weight conventions require reconciliation.

Mass = concentration × volume × molecular weight

MassConcentrationVolumeMolecular weightg/mol

Enter any three values to calculate the fourth.

C₁V₁ = C₂V₂

C₁mMV₁mLC₂mMV₂mL

Enter any three values to calculate the fourth.

Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass	1 mM	5 mM	10 mM
10 mg	16.7093 mL	3.3419 mL	1.6709 mL
25 mg	41.7732 mL	8.3546 mL	4.1773 mL
50 mg	83.5464 mL	16.7093 mL	8.3546 mL
100 mg	167.0928 mL	33.4186 mL	16.7093 mL
250 mg	417.7319 mL	83.5464 mL	41.7732 mL

Need a different pack size or technical document?

Contact our team with the catalog number and requested quantity.

Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?

We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.

Pure lipid or solution — which should I choose?

Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.

How should I measure a small amount of oily or viscous lipid?

Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.

Can you support formulation optimization and scale-up?

Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.

MOCHOL GMP-like Quality

Pack size & price

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