Product imageResearch use only

CAT. NO. DC33636Featured

DOTAP

Research highlights

DOTAP/cholesterol lipoplexmRNA transfectionThin-film hydration60-day refrigerated comparison

Pack size & price

USD

Add the cited formulation componentsSelected DOTAP pack + every supporting component at 100 mg each+US$450 / set

Supporting components

Cholesterol DCD-041 · 100 mg

Components are supplied separately; this is not a premixed LNP. RNA/cargo, buffer and formulation service are not included.

Total$100

We match the best price and quality on market.

Ships from ShanghaiIce-pack transportPure lipid recommended

Product overview

Description & Application

DOTAP, also known as 1,2-Dioleoyl-3-trimethylammoniumpropane, is a cationic liposome-forming compound used for transfection of DNA, RNA, and other negatively charged molecules into eukaryotic cells. It has been used in gene delivery vectors for gene ther

CAS No.	132172-61-3
Chemical Name	DOTAP
Synonyms	DOTAP; DOTAP chloride; N-(1-(2,3-Dioleoyloxy)propyl)-N,N,N-trimethylammonium chloride; (+/-)-Dotap chloride， DOTAP-Cl
SMILES	CCCCCCCC/C=C\CCCCCCCC(OC(C[N+](C)(C)C)COC(CCCCCCC/C=C\CCCCCCCC)=O)=O.[Cl-]
Formula	C42H80ClNO4
M.Wt	698.555
Purity	>95% by ELSD-HPLC
Storage	Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
Shipping condition	Ships from Shanghai. Pure lipid is stable during ice-pack transport.
Publication	Sun M, Dang UJ, Yuan Y, et al. Optimization of DOTAP/chol Cationic Lipid Nanoparticles for mRNA, pDNA, and Oligonucleotide Delivery. AAPS PharmSciTech. 2022;23:135.

Literature-reported formulation composition

Component	Reported molar ratio	Role
DOTAP	1	Ionizable lipid
Cholesterol	3	Sterol
Reported ratio sum	4	Original ratio retained

Component molecular weights and calculated amounts

Composition retained from the cited study below. Research planning aid only. Results cover lipid component weights and do not include RNA, buffer, solvent, process loss or an N/P ratio.

Total lipid mass (mg)

Component	Molecular weight	Molar parts	Normalized mol%	Amount (µmol)	Weight (mg)
DOTAP			25.000	53.8067	37.5869
Cholesterol			75.000	161.4201	62.4131

Calculated total: 100.0000 mg lipid.

Calculated catalog planning quantities; RNA, solvent and process loss are excluded. DMG-PEG2000 uses 2507. Component mass = total lipid mass × molar parts × molecular weight / sum(parts × molecular weight). This conversion does not infer N/P.

N/P ratioNot reported in the cited article

Total lipid:cargo weight ratioNot reported

Buffer & pH10 mM PBS, pH 7.4

CargoGFP-expressing mRNA

Administration routeIn vitro addition to complete cell-culture medium

Dose1 microgram mRNA with 62.5 micromolar total lipid

Animal modelSK-OV-3 human ovarian cancer cells, 100,000 cells per well

Formulation processDOTAP and cholesterol were dissolved in 2 mL chloroform at 10 micromoles total lipid, rotary-evaporated to a thin film, hydrated with 1 mL 10 mM PBS using repeated 30-second sonication and 60-second vortex cycles, extruded ten times through 200 nm and then 100 nm membranes, and complexed with 1 microgram mRNA for 30 minutes at room temperature.

Literature-reported performance for the cited formulation

Literature-reportedGFP-positive transfection efficiency49.4 ± 2.12%DOTAP/cholesterol 1:3 molar ratio, non-PEGylated, 62.5 micromolar total lipid, 1 microgram GFP mRNA · 24 h · JetPRIME 38.06 ± 3.68%; p = 0.017PMC full text, Results: In Vitro mRNA Transfection; Fig.4

Literature-reportedmRNA binding by gel retardationComplete binding for every tested non-PEGylated DOTAP/cholesterol ratio and lipid concentration1 microgram mRNA; DOTAP/cholesterol ratios 2:1, 1:1, 1:2, 1:3 and 1:4; total lipid 31.25, 62.5 or 125 micromolarPMC full text, Results: Gel Retardation Assay; Fig.2a

Literature-reportedRefrigerated mRNA-transfection retentionNo significant loss of transfection efficiency was reported through 60 days at 4 degrees CSelected non-PEGylated 1:3 formulation; compared over storage time · 0, 30 and 60 daysPMC full text, Stability Study; Fig.7

Literature Data DisclaimerAll formulation parameters and performance values shown in this section are derived from the cited publication and are provided for reference only. These results were obtained using the specific materials, formulation process, cargo, dose, analytical method, model and administration route described in that study. Unless otherwise stated, the data were not generated or independently verified by DC Chemicals. DC Chemicals supplies the lipid compound only and does not guarantee that customers will reproduce the reported LNP properties or biological performance.

The administration route shown above was used in the cited study and is not a clinical-use instruction or recommendation by DC Chemicals.

Cited literature for this formulation and performance datasetSun M, Dang UJ, Yuan Y, et al. Optimization of DOTAP/chol Cationic Lipid Nanoparticles for mRNA, pDNA, and Oligonucleotide Delivery. AAPS PharmSciTech. 2022;23:135. DOI: 10.1208/s12249-022-02294-w
Results are limited to the specified DOTAP/cholesterol lipoplex, preparation, cell line, cargo and exposure time.

Primary article

Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

The page calculator normalizes the reported 1:3 molar ratio to 25/75 mol% only for component-weight calculations. It does not calculate RNA amount or N/P.

Mass = concentration × volume × molecular weight

MassConcentrationVolumeMolecular weightg/mol

Enter any three values to calculate the fourth.

C₁V₁ = C₂V₂

C₁mMV₁mLC₂mMV₂mL

Enter any three values to calculate the fourth.

Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass	1 mM	5 mM	10 mM
10 mg	14.3153 mL	2.8631 mL	1.4315 mL
25 mg	35.7882 mL	7.1576 mL	3.5788 mL
50 mg	71.5763 mL	14.3153 mL	7.1576 mL
100 mg	143.1527 mL	28.6305 mL	14.3153 mL
250 mg	357.8816 mL	71.5763 mL	35.7882 mL

Need a different pack size or technical document?

Contact our team with the catalog number and requested quantity.

Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?

We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.

Pure lipid or solution — which should I choose?

Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.

How should I measure a small amount of oily or viscous lipid?

Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.

Can you support formulation optimization and scale-up?

Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.

DOTAP

Pack size & price

Description & Application

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