Literature-reported formulation composition
Component: DOTAP | Reported molar ratio: 1 | Role: Ionizable lipid
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DOTAP
Literature records
Search the tables and observations reproduced from the product literature guide. Rows may represent measurements, recipes or experimental context; they are not all independent experiments.
Literature-reported formulation composition
Component: Cholesterol | Reported molar ratio: 3 | Role: Sterol
Literature-reported formulation composition
Component: Reported ratio sum | Reported molar ratio: 4 | Role: Original ratio retained
Reported observation
Literature-reported GFP-positive transfection efficiency 49.4 ± 2.12% DOTAP/cholesterol 1:3 molar ratio, non-PEGylated, 62.5 micromolar total lipid, 1 microgram GFP mRNA · 24 h · JetPRIME 38.06 ± 3.68%; p = 0.017 PMC full text, Results: In Vitro mRNA Transfection; Fig.4
Reported observation
Literature-reported mRNA binding by gel retardation Complete binding for every tested non-PEGylated DOTAP/cholesterol ratio and lipid concentration 1 microgram mRNA; DOTAP/cholesterol ratios 2:1, 1:1, 1:2, 1:3 and 1:4; total lipid 31.25, 62.5 or 125 micromolar PMC full text, Results: Gel Retardation Assay; Fig.2a
Reported observation
Literature-reported Refrigerated mRNA-transfection retention No significant loss of transfection efficiency was reported through 60 days at 4 degrees C Selected non-PEGylated 1:3 formulation; compared over storage time · 0, 30 and 60 days PMC full text, Stability Study; Fig.7