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Ionizable lipids / Lipid 20b
Lipid 20b chemical structure
Product imageResearch use only
CAT. NO. DC60554Featured

Lipid 20b

Ionizable lipid for LNP and nucleic-acid delivery research.

Pack size & price

USD
TotalPrice on request
We match the best price and quality on market.
Ships from ShanghaiIce-pack transportPure lipid recommended
Product overview

Description & Application

Lipid 20b​​ is a thiophene-based ionizable lipid synthesized via the Gewald reaction. It features dual unsaturated linoleic tails (C18:2) attached to the same side of the thiophene core and a tertiary amine headgroup. Formulated into LNPs (~100 nm, PDI ~0.2) with DSPC/cholesterol/DMG-PEG, it exhibits high mRNA encapsulation (>90%). Unlike traditional lipids, 20b lacks a pH-dependent ionization profile, likely due to electron delocalization in the thiophene ring. Intravenously, 20b LNPs transfect the liver and spleen in mice. Notably, subretinal delivery in mice and non-human primates (NHPs) achieved robust mRNA expression in photoreceptors (35% rods, 45% cones at high dose) and retinal pigment epithelium (RPE) with minimal acute toxicity. Immunosuppression enhanced rod transfection efficiency. High-dose administration in NHPs caused subretinal debris, but low doses (2.5 µg mRNA) maintained retinal health. This lipid demonstrates potential for liver and retinal gene therapy.

CAS No.Not available
Chemical NameLipid 20b
SynonymsLipid-20b
SMILESNot available
FormulaC44H72N2O3S
M.Wt709.12
Purity>98%
StoragePure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
Shipping conditionShips from Shanghai. Pure lipid is stable during ice-pack transport.
PublicationThiophene-based lipids for mRNA delivery to pulmonary and retinal tissues-PNAS-March 4, 2024 121 (11) e2307813120
Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

Mass = concentration × volume × molecular weight
Enter any three values to calculate the fourth.
C₁V₁ = C₂V₂
Enter any three values to calculate the fourth.
Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass1 mM5 mM10 mM
10 mg14.1020 mL2.8204 mL1.4102 mL
25 mg35.2550 mL7.0510 mL3.5255 mL
50 mg70.5099 mL14.1020 mL7.0510 mL
100 mg141.0199 mL28.2040 mL14.1020 mL
250 mg352.5496 mL70.5099 mL35.2550 mL

Need a different pack size or technical document?

Contact our team with the catalog number and requested quantity.

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Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?
We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.
Pure lipid or solution — which should I choose?
Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.
How should I measure a small amount of oily or viscous lipid?
Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.
Can you support formulation optimization and scale-up?
Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.