Product imageResearch use only

CAT. NO. DC57002Featured

LIPID C24

Research highlights

48/13/37/2 mol% formulationN/P 4; TNS apparent pKa 6.7780 nm reported particle sizeLow-dose intramuscular mRNA vaccine study

Pack size & price

USD

Add the cited formulation componentsSelected LIPID C24 pack + every supporting component at 100 mg each+US$450 / set

Supporting components

DSPC DC57005 · 100 mg+Cholesterol DCD-041 · 100 mg+DMG-PEG2000 DC40169 · 100 mg

Components are supplied separately; this is not a premixed LNP. RNA/cargo, buffer and formulation service are not included.

Total$400

We match the best price and quality on market.

Ships from ShanghaiIce-pack transportPure lipid recommended

Product overview

Description & Application

C24 is a novel multiprotic ionizable lipid. C24 lipid nanoparticle (LNP) has a multistage protonation behavior resulting in greater endosomal protonation and greater translation compared to the standard reference MC3 LNP. C24 LNP also lower injection site inflammation and higher stability compared to MC3 LNP.

CAS No.	2767561-52-2
Chemical Name	LIPID C24
Synonyms	C24,C-24,C 24
SMILES	N(CCC(OCC(CCCCCCCC)CCCCCCCCCC)=O)(CCC(OCC(CCCCCCCC)CCCCCCCCCC)=O)CCCCN1CCN(C)CC1
Formula	C55H109N3O4
M.Wt	875.84
Purity	>95% by ELSD-HPLC
Storage	Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
Shipping condition	Ships from Shanghai. Pure lipid is stable during ice-pack transport.
Publication	Alishetty S, et al. Novel lipid nanoparticle provides potent SARS-CoV-2 mRNA vaccine at low dose with low local reactogenicity, high thermostability and limited systemic biodistribution. Research Square. 2021.

Literature-reported formulation composition

Component	mol%	Role
C24	48	Ionizable lipid
DSPC	13	Helper phospholipid
Cholesterol	37	Sterol
DMG-PEG2000	2	PEG lipid
Reported total	100	Source values retained

Component molecular weights and calculated amounts

Composition retained from the cited study below. Research planning aid only. Results cover lipid component weights and do not include RNA, buffer, solvent, process loss or an N/P ratio.

Total lipid mass (mg)

Component	Normalized mol%	Amount (µmol)	Weight (mg)
C24	48.000	67.0089	58.6890
DSPC	13.000	18.1482	14.3398
Cholesterol	37.000	51.6527	19.9715
DMG-PEG2000	2.000	2.7920	6.9996

Calculated total: 100.0000 mg lipid.

Calculated catalog planning quantities; RNA, solvent and process loss are excluded. DMG-PEG2000 uses 2507. Component mass = total lipid mass × molar parts × molecular weight / sum(parts × molecular weight). This conversion does not infer N/P.

N/P ratio4

Total lipid:cargo weight ratioNot reported and not inferred.

Buffer & pHPBS pH 7.4 during collection and dilution; final dialysis to pH 7.3-7.4.

CargoFirefly-luciferase mRNA or SARS-CoV-2 S2P mRNA, according to experiment

Administration routeIntramuscular

DoseReporter studies: 0.5 or 5 micrograms mRNA. Immunogenicity/challenge: 0.1, 0.25, 0.5 or 1 microgram S2P mRNA.

Animal modelBALB/c mice and K18-hACE2 mice

Formulation processLipids in ethanol and mRNA in aqueous buffer were mixed with a Spark NanoAssemblr. The dispersion was ejected into PBS, diluted 1:1 with PBS and dialyzed with six buffer exchanges over 6 h using a 10 kDa device. Exact stream flow rates were not reported.

Literature-reported performance for the cited formulation

Literature-reportedTNS apparent pKa6.77C24 LNP; MC3 comparator 6.55PDF Results and Fig. 1

Literature-reportedZeta-potential fit pKa / isoelectric point5.21 / 6.12Separate electrokinetic titration; not interchangeable with TNS pKaPDF Results and Fig. 1

Literature-reportedRiboGreen-inaccessible mRNA68%C24 LNP; MC3 79%; not labeled as definitive EEPDF Results and Fig. 1

Literature-reportedMuscle luciferase expressionApproximately 4-fold MC30.5 microgram mRNA IM; 4 hPDF Results and Fig. 2

Literature-reportedPseudovirus neutralizationApproximately 10-fold MC3 after boost0.25-1 microgram S2P mRNA dosesPDF Results and Fig. 4

Literature-reportedChallenge protection3/3 followed mice survived at 0.25 microgramK18-hACE2; initial n=5, two animals/group used for day-5 lung titersPDF Fig. 5 legend

Selected C24 study findings

Experiment	Condition	Reported result
Reporter expression	0.5 microgram mRNA IM; 4 h	Approximately 4-fold MC3 at muscle
Local histology	5 micrograms mRNA; 24 h; n=3	Less injection-site inflammation than MC3 in this study
K18-hACE2 challenge	0.25 microgram S2P; survival n=3	3/3 followed mice survived
Short-term storage	PBS; 14 days	Bioactivity stable at 4 C; 20-40% decrease at room temperature

Preprint Figs. 2-6 and supplementary stability figures.

Additional reported findings

Liver off-target reporter signal

At 5 micrograms mRNA, liver signal was reported as approximately sixfold lower than MC3 (p=0.051).

BALB/c IM reporter study · PDF Results and Fig. 2

Lung viral titers

C24 at 0.5 microgram eliminated detectable lung infection in the two animals assessed on day 5; MC3 did not completely eliminate infection even at 1 microgram.

K18-hACE2 challenge; n=2 for lung titers · PDF Fig. 5

RNA integrity

mRNA integrity was retained for at least 19 days at 4 C and declined more slowly for C24 than MC3 at higher storage temperatures.

Study formulation in PBS · Supplementary stability figures

Literature Data DisclaimerAll formulation parameters and performance values shown in this section are derived from the cited publication and are provided for reference only. These results were obtained using the specific materials, formulation process, cargo, dose, analytical method, model and administration route described in that study. Unless otherwise stated, the data were not generated or independently verified by DC Chemicals. DC Chemicals supplies the lipid compound only and does not guarantee that customers will reproduce the reported LNP properties or biological performance.

The administration route shown above was used in the cited study and is not a clinical-use instruction or recommendation by DC Chemicals.

Cited literature for this formulation and performance datasetAlishetty S, et al. Novel lipid nanoparticle provides potent SARS-CoV-2 mRNA vaccine at low dose with low local reactogenicity, high thermostability and limited systemic biodistribution. Research Square. 2021. DOI: 10.21203/rs.3.rs-798453/v1
All formulation and performance claims are restricted to the reported C24 formulations and stated experimental conditions.

Research Square preprint

Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

Lipid component weights only. DMG-PEG2000 uses the website-standard editable MW 2507. RNA, buffer, solvent, process loss and N/P are not included in the lipid-component total.

Mass = concentration × volume × molecular weight

MassConcentrationVolumeMolecular weightg/mol

Enter any three values to calculate the fourth.

C₁V₁ = C₂V₂

C₁mMV₁mLC₂mMV₂mL

Enter any three values to calculate the fourth.

Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass	1 mM	5 mM	10 mM
10 mg	11.4176 mL	2.2835 mL	1.1418 mL
25 mg	28.5440 mL	5.7088 mL	2.8544 mL
50 mg	57.0881 mL	11.4176 mL	5.7088 mL
100 mg	114.1761 mL	22.8352 mL	11.4176 mL
250 mg	285.4403 mL	57.0881 mL	28.5440 mL

Need a different pack size or technical document?

Contact our team with the catalog number and requested quantity.

Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?

We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.

Pure lipid or solution — which should I choose?

Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.

How should I measure a small amount of oily or viscous lipid?

Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.

Can you support formulation optimization and scale-up?

Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.

LIPID C24

Pack size & price

Description & Application

Recommended Products

DLin-MC3-DMA

DLinDMA

DLin-KC2-DMA

E10i-494

LP-01

SM-86(Lipid 5)

DODMA

DODAP

DOTAP

DMHAPC-Chol