For research use only. We do not sell to patients.
Ionizable lipids / C12-2aN
C12-2aN chemical structure
Product imageResearch use only
CAT. NO. DC60978Featured

C12-2aN

Ionizable lipid for LNP and nucleic-acid delivery research.

Pack size & price

USD
TotalPrice on request
We match the best price and quality on market.
Ships from ShanghaiIce-pack transportPure lipid recommended
Product overview

Description & Application

C12-2aN is a crosslinked ionizable lipid developed for mRNA vaccine delivery and dendritic-cell metabolic reprogramming. Its structure combines two piperazine-based ionizable amine cores, a bis-amidine crosslinker, and four hydroxylated C12 hydrophobic tails. When formulated with DOPE, cholesterol, and C14-PEG2000, C12-2aN LNPs enhanced mRNA endosomal escape and activated AMPK–mTORC2-dependent glycolysis, supporting dendritic-cell maturation and antigen presentation. In preclinical mouse studies, C12-2aN LNPs generated robust humoral and cellular immune responses in SARS-CoV-2 RBD and OVA cancer-vaccine models. The formulation also demonstrated reduced liver-associated off-target expression and lower acute inflammatory markers than the tested control formulations. C12-2aN is a preclinical research lipid intended for evaluating metabolically active mRNA vaccine delivery systems.

CAS No.Not available
Chemical NameC12-2aN
SynonymsNot available
SMILESCCCCCCCCCCC(O)CN(CCCN1CCN(CCCNC(=N)CCCCC(=N)NCCCN2CCN(CCCN(CC(O)CCCCCCCCCC)CC(O)CCCCCCCCCC)CC2)CC1)CC(O)CCCCCCCCCC
FormulaC₇₄H₁₅₂N₁₀O₄
M.Wt1,246.0960
Purity98%
StoragePure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
Shipping conditionShips from Shanghai. Pure lipid is stable during ice-pack transport.
PublicationKim D, Gong N, Alameh M-G, Han EL, Wang H, Wang J, Feng E, Yoon I-C, Murray AM, Shi Q, Moon S-J, Mrksich K, Weissman D, Mitchell MJ. Crosslinked ionizable lipids reprogram dendritic cell metabolism for potent mRNA vaccination. Nature Materials. 2026.
Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

Mass = concentration × volume × molecular weight
Enter any three values to calculate the fourth.
C₁V₁ = C₂V₂
Enter any three values to calculate the fourth.
Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass1 mM5 mM10 mM
10 mg8.0251 mL1.6050 mL802.51 μL
25 mg20.0627 mL4.0125 mL2.0063 mL
50 mg40.1253 mL8.0251 mL4.0125 mL
100 mg80.2506 mL16.0501 mL8.0251 mL
250 mg200.6266 mL40.1253 mL20.0627 mL

Need a different pack size or technical document?

Contact our team with the catalog number and requested quantity.

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Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?
We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.
Pure lipid or solution — which should I choose?
Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.
How should I measure a small amount of oily or viscous lipid?
Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.
Can you support formulation optimization and scale-up?
Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.