Product imageResearch use only

CAT. NO. DC68150Featured

Lipid A10 (CSS-A10)

Research highlights

Ionizable lipid used in LNP67Bone-marrow HSC and HSPC deliveryRhesus CD34+ cell mRNA deliveryApparent LNP pKa 6.4

Pack size & price

USD

Total$950

We match the best price and quality on market.

Ships from ShanghaiIce-pack transportPure lipid recommended

Product overview

Description & Application

Lipid A10 (CSS-A10) is a diketopiperazine-core ionizable lipid used in the LNP67 formulation for mRNA delivery to hematopoietic stem and progenitor cells. In the cited study, ligand-free LNP67 delivered reporter mRNA to mouse bone-marrow HSC/HSPC populations and to CD34+ cells in young rhesus monkeys after intravenous administration.

CAS No.	Not available
Chemical Name	Lipid A10 (CSS-A10)
Synonyms	CSS-A10, Lipid A10, A10
SMILES	CCCCCCCCCCN(CCCCCCCCCC)CCC(=O)OC[C@@H]1NC(=O)[C@H](COC(=O)CCN(CCCCCCCCCC)CCCCCCCCCC)NC1=O
Formula	C52H100N4O6
M.Wt	877.39
Purity	>95% by ELSD-HPLC
Storage	Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
Shipping condition	Ships from Shanghai. Pure lipid is stable during ice-pack transport.
Publication	Kim H, Zenhausern R, Gentry K, et al. Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys. Nature Biotechnology. Published online 16 November 2024. Hatit MZC, Ni H, Dahlman JE, et al. Novel lipids containing diketopiperazine core, lipid nanoparticle containing thereof, and methods of using thereof. WO2025217298A1. Published 16 October 2025.

Literature-reported formulation composition

Component	mol%	Role
CSS-A10	35	Ionizable lipid
Cholesterol	47.5	Sterol
C14PEG2000	2.5	PEG lipid
DOTAP	15	Additional lipid components
Reported total	100	Source values retained

Formulation calculator unavailable. CSS-A10 is verified as C52H100N4O6 with a calculated average MW of 877.39 g/mol. The exact molecular-weight specifications for C14PEG2000 and DOTAP remain unverified in this formulation record. Obtain and verify the complete composition and component molecular weights before planning component quantities.

N/P ratioNot reported in the cited article

Buffer & pHmRNA diluted in 25 mM sodium acetate; lipid phase prepared in ethanol; final LNP dialyzed into sterile PBS. The rhesus formulation was cryopreserved in 10% sucrose/PBS before shipment and dosing.

CargoMembrane-anchored NanoLuc luciferase mRNA (aNLuc mRNA)

Administration routeIntravenous injection

Dose0.25 or 0.4 mg mRNA/kg, single dose

Animal modelYoung rhesus monkeys (Macaca mulatta), 7-8 months old; n=4 at 0.25 mg/kg and n=3 at 0.4 mg/kg; one untreated control; tissues collected approximately 24 h after dosing

Formulation processAqueous nucleic-acid and ethanol lipid phases were mixed using a NanoAssemblr Spark or Ignite at a 3:1 aqueous:lipid-phase flow-rate ratio, dialyzed against PBS overnight at 4 °C and sterile-filtered. The NHP batch was frozen, shipped, thawed and tested before dosing.

Literature-reported performance for the cited formulation

Literature-reportedHydrodynamic diameter80 nmLNP67 characterizationMain article Figure 2d

Literature-reportedmRNA encapsulation efficiency86.7%LNP67 characterizationMain article Figure 2b

Literature-reportedZeta potential-2.58 mVLNP67 characterizationMain article Figure 2e

Literature-reportedApparent LNP pKa6.4TNS fluorescence assayMain article Figure 2f and Methods

Literature-reportedFunctional mRNA delivery to rhesus CD34+ cellsDose-dependent aNLuc expression was detected in CD34+ bone-marrow cells; expression in CD34+ cells was similar to liver-cell expression at both tested doses.Approximately 24 h · Untreated control and liver-cell suspensionsMain article Figure 4c-g and accompanying text

Additional reported findings

Ligand-free bone-marrow delivery

LNP67 was selected from an in vivo screen of 105 quality-controlled formulations and showed the most favorable bone-marrow-versus-liver delivery profile among the individually tested lead formulations.

Mouse screening and individual confirmation; no antibody or other targeting ligand was attached to LNP67 · Main article Figures 1g and 2a

Mouse HSC/HSPC dose response

After intravenous administration, LNP67 produced dose-dependent reporter expression across eight bone-marrow HSC/HSPC subtypes, including reporter-positive multipotent progenitors.

C57BL/6J mice; aVHH mRNA; 0.5, 1.0 and 1.5 mg/kg; 16 h · Main article Figure 2i

Preliminary rhesus tolerability observation

The authors reported no adverse findings or serum-cytokine differences versus the untreated control at the tested doses without anti-inflammatory pretreatment.

Seven treated young rhesus monkeys and one untreated control; 23 serum cytokines; approximately 24 h. This small study is not a formal toxicology package. · Main article Figure 4h and Discussion

Literature Data DisclaimerAll formulation parameters and performance values shown in this section are derived from the cited publication and are provided for reference only. These results were obtained using the specific materials, formulation process, cargo, dose, analytical method, model and administration route described in that study. Unless otherwise stated, the data were not generated or independently verified by DC Chemicals. DC Chemicals supplies the lipid compound only and does not guarantee that customers will reproduce the reported LNP properties or biological performance.

The administration route shown above was used in the cited study and is not a clinical-use instruction or recommendation by DC Chemicals.

Cited literature for this formulation and performance datasetKim H, Zenhausern R, Gentry K, et al. Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys. Nature Biotechnology. Published online 16 November 2024. DOI: 10.1038/s41587-024-02470-2
The Nature Biotechnology article reports LNP67 composition by component class, physicochemical properties and mouse/rhesus performance, while calling the ionizable lipid proprietary. WO2025217298A1, claiming priority to US 63/632,354 cited by the paper, identifies LNP67 as CSS-A10/cholesterol/C14PEG2000/DOTAP at 35:47.5:2.5:15 mol%.

Primary article · LNP67 patent disclosure

Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

Molecular formula: C52H100N4O6. Molecular weight: 877.39. Configuration: (2S,5S). Source identity: WO2025217298A1, Example 7, Compound 16.

Mass = concentration × volume × molecular weight

MassConcentrationVolumeMolecular weightg/mol

Enter any three values to calculate the fourth.

C₁V₁ = C₂V₂

C₁mMV₁mLC₂mMV₂mL

Enter any three values to calculate the fourth.

Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass	1 mM	5 mM	10 mM
10 mg	11.3974 mL	2.2795 mL	1.1397 mL
25 mg	28.4936 mL	5.6987 mL	2.8494 mL
50 mg	56.9872 mL	11.3974 mL	5.6987 mL
100 mg	113.9744 mL	22.7949 mL	11.3974 mL
250 mg	284.9360 mL	56.9872 mL	28.4936 mL

Need a different pack size or technical document?

Contact our team with the catalog number and requested quantity.

Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?

We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.

Pure lipid or solution — which should I choose?

Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.

How should I measure a small amount of oily or viscous lipid?

Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.

Can you support formulation optimization and scale-up?

Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.

Lipid A10 (CSS-A10)

Pack size & price

Description & Application

Recommended Products

KC-34(SPC-A9)

C14-490

Lipid-168

Lipid H5T5

Generation Lipid 87

lipid a12Dab4

A2-Iso5-2DC18

E12CA1A3

YHS-12

CICL-207