DC Chemicals · Product datasheet

DBeQ — Datasheet

CAT. NO. DC7602

Product page and current package options

ParameterValue
Chemical NameN,N'-Dibenzylquinazoline-2,4-diamine
CAS No.177355-84-9
SynonymsDBeq;N,N'-Dibenzyl-2,4-quinazolinediamine;JRF 12;JRF-12;N,N'-Dibenzylquinazoline-2,4-diamine;JRF;N2,N4-Dibenzylquinazoline-2,4-diamine;N,N'-Dibenzylquinazoline-2,4-diamine DBeQ;2,4-QuinazolinediaMine, N,N'-bis(phenylMethyl)-;2,4-QuinazolinediaMine, N2,N4-bis(phenylMethyl)-;N2,N4-Dibenzylquinazoline-2,4-diamine DBeQ;2-N,4-N-dibenzylquinazoline-2,4-diamine;KUC105555N;C22H20N4;MLS000769571;SMR002239188;SMR000434303;N*2*,N*4*-Dibenzyl-quinazoline-2,4-diamine;N2,N4-Bis(phenylmethyl)-2,4-quinazolinediamine;2,4-dibencilaminoquinazoline;JRF12;Oprea1_067556;Oprea1_526582;MLS006012041;MLS003568133;BDBM46584
FormulaC22H20N4
M.Wt340.421
SMILESN([H])(C([H])([H])C1C([H])=C([H])C([H])=C([H])C=1[H])C1C2=C([H])C([H])=C([H])C([H])=C2N=C(N=1)N([H])C([H])([H])C1C([H])=C([H])C([H])=C([H])C=1[H]
Purity>98%
Storage2 years -20°C Powder, 2 weeks 4°C in DMSO, 6 months -80°C in DMSO

Biological Activity

DBeQ is a selective, potent, reversible, and ATP-competitive p97 inhibitor, with an IC50 value of 1.5 μM and 1.6 μM for p97(wt) and p97(C522A), respectively; DBeQ also inhibits Vps4 with an IC50 of 11.5 μM.

In Vitro

DBeQ is a ATP-competitive p97 inhibitor, with an IC50 value of 1.5 μM and 1.6 μM for p97(wt) and p97(C522A), respectively. DBeQ inhibits p97 competitively with respect to ATP, with a Ki of 3.2 ± 0.4 μM. DBeQ inhibits degradation of the p97-dependent substrate UbG76V-GFP, with IC50 value of 2.6 μM. DBeQ (10 μM) also significantly suppresses degradation of TCRα-GFP, induces CHOP but does not increase p21 level. Moreover, DBeQ inhibits the viability of MRC-5, Hek293, HeLa and RPMI8226 cells, with GI50s of 6.6 ± 2.9, 4 ± 0.6, 3.1 ± 0.5 and 1.2 ± 0.3, respectively[1]. DBeQ potently inhibits the AAA ATPase p97 by specifically binding to the ATPase site of its D2 domain (p97D2). DBeQ also inhibits Vps4, with an IC50 of 11.5 μM. Furthermore, DBeQ (30 μM) inhibits hyphal growth of the wild-type cell (strain YLZ0)[2].

In Vivo

No in vivo data are provided in the current product source. Contact us for further information.

References & source

DC Chemicals original product information

Published experimental findings depend on the model and assay conditions. They do not guarantee equivalent performance in every application. For laboratory research use only.

Cart

Request quotation