Biological Activity
Lats-IN-1 is a potent and ATP-competitive inhibitor of Lats1 and Lats2 kinases. Lats-IN-1 promotes Yap-dependent proliferation in postmitotic mammalian tissues[1].
In Vitro
The IC50 for Lats-IN-1 increases with the ATP concentration[1]. Lats-IN-1 (10 μM; 24 hours) interferes with the ability of Lats kinases to phosphorylate Yap, with an EC50 of 510 nM[1]. Lats-IN-1 causes Yap-dependent proliferation of murine supporting cells in the inner ear, murine cardiomyocytes, and human Müller glia in retinal organoids[1]. Lats-IN-1 fosters both the G1-S and G2-M checkpoint transitions and yields supporting cells capable of transdifferentiation[1]. Cell Proliferation Assay[1] Cell Line: MCF 10A cells Concentration: 10 μM Incubation Time: 24 hours Result: Decreased the phosphorylation of Yap at residue S127.
In Vivo
No in vivo data are provided in the current product source. Contact us for further information.
References & source
Published experimental findings depend on the model and assay conditions. They do not guarantee equivalent performance in every application. For laboratory research use only.