Biological Activity
D159687 is a selective PDE4D inhibitor[1].
In Vitro
D159687 (1 μM, 0-24 hours) induces a transient increase in CREB phosphorylation which peaked at 6 hours after treatment[1]. D159687 (0.01-1 μM, 6 hours) causes optimal CREB phosphorylation at 1 μM[1]. Western Blot Analysis[1] Cell Line: HT-22 (mouse hippocampal cell line) Concentration: 1 μM Incubation Time: 0, 1, 3, 6, 12, 24 hours Result: Induced a transient increase in CREB phosphorylation which peaked at 6 hours after treatment. Western Blot Analysis[1] Cell Line: HT-22 (mouse hippocampal cell line) Concentration: 0.01 μM, 0.1 μM, 1 μM Incubation Time: 6 hours Result: CREB phosphorylation was optimal at 1 μM.
In Vivo
D159687 (0.05-5 mg/kg; oral daily for a week) shows a potential recruitment or enhancement of synaptic function with increased task difficulty in female Cynomolgus macaques[2]. Animal Model: Female Cynomolgus macaques (4–6 year old) [2] Dosage: 0.05, 0.5, 5 mg/kg Administration: Oral daily for a week Result: A potential recruitment or enhancement of synaptic function with increased task difficulty.
References & source
Published experimental findings depend on the model and assay conditions. They do not guarantee equivalent performance in every application. For laboratory research use only.