Biological Activity
PD 404182 is a potent and competitive inhibitor of human dimethylarginine dimethylaminohydrolase 1 (DDAH1), with an IC50 of 9 μM. PD 404182 exhibits antiangiogenic and antiviral activity in vitro[1][2].
In Vitro
PD 404182 (20 μM) increases asymmetric dimethylarginine (ADMA) approximately 70% in ECs[1]. PD 404182 (50-100 μM; 18 hours) attenuates endothelial tube formation in virto and does not perturb cell membrane integrity or induces cytotoxicity[1]. PD 404182 inhibits HIV-1 in seminal plasma, with an IC50 of 1 μM[2]. PD 404182 (300 μM;30 minutes) exhibits low toxicity toward several human cell lines , freshly activated PBMCs, primary CD4+ T lymphocytes, macrophages, and dendritic cells, and lactobacilli found in the normal vaginal flora[2]. Cell Viability Assay[1] Cell Line: Human dermal microvascular endothelial cells (ECs) Concentration: 10-300 μM Incubation Time: 24 hours Result: Did not induce cytotoxicity.
In Vivo
No in vivo data are provided in the current product source. Contact us for further information.
References & source
Published experimental findings depend on the model and assay conditions. They do not guarantee equivalent performance in every application. For laboratory research use only.