Product imageResearch use only

CAT. NO. DCL0002

HI-62

Cyclic-thiourea ionizable lipid for mRNA-LNP and vaccine research.

Research highlights

E21 particle size: 89.3 ± 0.6 nm; PDI: 0.03.Reported mRNA encapsulation: 97.5%; apparent particle pKa: 6.45.At 48 h after intravenous administration, reporter signal remained more than three orders of magnitude above the SM-102 comparator.Complete tumor responses were reported in 4 of 5 mice in the cited TC-1 model.

Literature- or patent-reported values; not product specifications or performance guarantees.

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Research use only

Pack sizes, price, stock status and batch documents are confirmed individually.

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Product overview

Description & Application

HI-62 is a cyclic-thiourea ionizable lipid reported as a lead component of onion-like multilamellar mRNA-LNPs. In the cited preprint, its E21 formulation supported high mRNA encapsulation, sustained reporter expression, frozen-storage stability and antitumor activity in a preclinical mRNA-vaccine model.

CAS No.	Not available
Chemical Name	HI-62
SMILES	CCCCCCCCC(CC)OC(=O)CCCCCCCN(CCCCCCCC(=O)OC(CCCCCCCC)CCCCCCCC)CCCN1CNC(=S)NC1
Formula	C50H98N4O4S
M.Wt	851.42
Purity	Contact us for the current batch specification and COA.
Storage	Pure form: store at −20 °C, sealed and protected from light. Contact us for batch-specific handling guidance.
Shipping condition	Ships from Shanghai. Contact us to confirm the appropriate transport condition for the current batch.
Product classification	Cationic/Ionizable Lipids; Ionizable Lipids for RNA Cancer Vaccines

Literature-reported formulation and performance

Formulation IDE21

Particle size89.3 ± 0.6 nm

PDI0.03

Encapsulation efficiency97.5%

Apparent particle pKa6.45 (TNS fluorescence assay)

N/P ratio3–7 tested; approximately 97% encapsulation at N/P 3

Mixing methodMicrofluidic mixing; organic:aqueous volume ratio 1:3; total flow rate 20 mL/min

Administration routeIntravenous or intramuscular, depending on the study module

Literature-reportedReporter expression>3 orders of magnitude above SM-102 at 48 h5 µg FLuc mRNA per mouse; intravenous study

Literature-reportedFreeze–thaw encapsulation>95% after six cyclesSM-102 comparator decreased from 96% to below 90%

Literature-reportedTC-1 tumor responseComplete responses in 4 of 5 mice10 µg mRNA per dose on days 0, 5 and 10

Literature Data DisclaimerThese values apply only to the cited formulation, process, cargo, dose and study model. They are not product release specifications or a guarantee of performance.

Cited literatureZhang L, Huang Z, et al. Cyclic-thiourea ionizable lipids program multilamellar mRNA-LNPs for sustained expression, frozen-storage stability and potent vaccine immunotherapy. bioRxiv, posted 9 September 2026. DOI: 10.64898/2026.09.07.749752

SDSMSDSAvailable upon requestRequest document →

COACOABatch-specific certificateRequest document →

DATADatasheetProduct information pageRead online →

Laboratory planning

Solution Calculators

The product molecular weight is prefilled when available and remains editable. Confirm solvent compatibility and solubility before preparation.

Mass = concentration × volume × molecular weight

MassConcentrationVolumeMolecular weightg/mol

Enter any three values to calculate the fourth.

C₁V₁ = C₂V₂

C₁mMV₁mLC₂mMV₂mL

Enter any three values to calculate the fourth.

Preparing stock solutions

Quick preparation table

Solvent volumes update from the molecular weight above.

Mass	1 mM	5 mM	10 mM
10 mg	11.6504 mL	2.3301 mL	1.1650 mL
25 mg	29.1260 mL	5.8252 mL	2.9126 mL
50 mg	58.2520 mL	11.6504 mL	5.8252 mL
100 mg	116.5039 mL	23.3008 mL	11.6504 mL
250 mg	291.2599 mL	58.2520 mL	29.1260 mL

Need a different pack size or technical document?

Contact our team with the catalog number and requested quantity.

Handling guidance

Shipping, storage and sampling

How is the lipid shipped, and how should I store it?

We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results.

Pure lipid or solution — which should I choose?

Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed.

How should I measure a small amount of oily or viscous lipid?

Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first.

Can you support formulation optimization and scale-up?

Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.