BP-200-C12, BP-197-C12 and BP-488-C10 were top in the BJ fibroblast luciferase-mRNA screen. BP-200-C12 was selected for subsequent studies (Figure 1c–e).
| CAS No. | Not available |
| Chemical Name | BP-200-C12 |
| SMILES | O=P(O)(O)C(O)(CCCN(C(=O)CN(CC(O)CCCCCCCCCC)CCN1CCN(CCN(CC(O)CCCCCCCCCC)CCN(CC(O)CCCCCCCCCC)CC(O)CCCCCCCCCC)CC1)CCC(=O)N2CCN(C(=O)CN(CC(O)CCCCCCCCCC)CCN3CCN(CCN(CC(O)CCCCCCCCCC)CCN(CC(O)CCCCCCCCCC)CC(O)CCCCCCCCCC)CC3)CC2)P(=O)(O)O |
| Formula | C131H267N13O18P2 |
| M.Wt | 2374.60 |
| Purity | >95% by ELSD-HPLC |
| Storage | Confirm with batch-specific handling documentation |
| Product classification | Cationic/Ionizable Lipids; Ionizable Lipids for mineralized tissue RNA delivery |
| Publication | Liu J, et al. Chemically Tailored Lipids Mediate Solid-phase RNA Programming of Mineralized Microenvironments. Biomaterials (2026), article 124680. doi:10.1016/j.biomaterials.2026.124680 |
Published BP-200-C12 LNP formulation
| Component | mol% | Role |
|---|---|---|
| BP-200-C12 | 35 | Ionizable lipid |
| DOPE | 16 | Helper phospholipid |
| Cholesterol | 46.5 | Sterol |
| C14-PEG2000 PE | 2.5 | PEG lipid |
| Total | 100 | Reported molar ratio |
Reported measurements and study results
After 120 min, fluorescently labeled MiTEX showed 80% HA binding versus 10% for the C12-200 LNP comparator (Figure 2c,d). The lipid and whole LNP are distinct.
Following 2,000 ng input mRNA and substrate washout, about 200 ng mRNA remained on HA in the MiTEX condition versus less than 50 ng in the control (Figure 3b). Cre-mRNA-loaded HA labeled approximately 80% of tdROSA mMSCs versus 17.92% with the control (Figure 3e,f).
Cre-mRNA-loaded HA/TCP with donor mMSCs was implanted subcutaneously in mice and assessed at 60 days (Figure 4). Separately, STAT3-siRNA MiTEX reduced pSTAT3 and IL-8 in HA-associated gingival fibroblast experiments; the human explant IL-6 readout was significant while IL-8 was not (Figure 5).
Primary publication: Liu J, et al. Biomaterials (2026), article 124680. Structure and spectroscopy: Supplementary Figure S1a-d (BP-200-C12); amine core and C12 epoxide: main Figure 1b. Composition: main Figure 1 and Methods; results: main Figures 1-5. The formula and molecular weight are calculated from the fully protonated phosphonic-acid drawing; the paper does not report a measured mass for this final compound. doi:10.1016/j.biomaterials.2026.124680