79.6 nm Z-average diameter, PDI 0.134, 99.40% mRNA encapsulation and zeta potential +1.13 mV (Supplementary Table S1). These characterize the formulated LNP, not the neat lipid.
| CAS No. | N/A |
| Chemical Name | Lipid 6 |
| Synonyms | Lipid 6; amine-core-6 lipidoid |
| SMILES | N(CC(O)CCCCCCCCCCCC)(CC(O)CCCCCCCCCCCC)CCOCCN(CCOCCN(CC(O)CCCCCCCCCCCC)CC(O)CCCCCCCCCCCC)CCN1CCN(CCOCCN(CC(O)CCCCCCCCCCCC)CC(O)CCCCCCCCCCCC)CC1 |
| Formula | C102H210N6O9 |
| M.Wt | 1664.84 |
| Purity | >95 by ELSD-HPLC |
| Storage | Confirm with batch-specific handling documentation |
| Product classification | Cationic/Ionizable Lipids; Ionizable Lipids for ex vivo CAR-T research |
| Publication | Gao H, et al. Lipid-based mRNA chimeric antigen receptor T cells for enhanced in vitro antitumor activity. iScience. 2026;29:117390. doi:10.1016/j.isci.2026.117390 |
Published Lipid 6 LNP compositions
| Component | Initial screen (mol%) | Selected B13 (mol%) |
|---|---|---|
| Lipid 6 | 35 | 30.00 |
| DOPE | 16 | 15.00 |
| Cholesterol | 46.5 | 53.13 |
| DMG-PEG2000 | 2.5 | 1.88 |
| Reported total | 100 | 100.01 (rounded SI values) |
Initial screening formulation: main text, page 2. B13: main text, page 5 and Supplementary Table S2. B13 was selected for follow-up T-cell experiments because it combined high mean fluorescence intensity with a lower PEG-lipid fraction than B11.
Reported measurements and biological results
With the optimized lipid-6 protocol, >90% eGFP-positive dendritic and NK cells and >97% eGFP-positive tumor-infiltrating lymphocytes were reported. Activated primary T cells exceeded 95%; nonactivated T cells exceeded 80% (Figure 4; three donors).
Approximately 90% CAR-positive primary human T cells 24 h after transfection with lipid-6 LNPs carrying MUC1-CAR mRNA (Figure 5C). The engineered cells showed greater in vitro killing of MUC1-positive MDA-MB-468 and SKOV3 cells than controls in the reported co-culture assays (Figures 6 and 7; three donors). This is not an in vivo CAR-T efficacy result.
After 0.5 mg/kg firefly luciferase mRNA, lipid-6 LNPs produced higher bioluminescence than SM-102 and ALC-0315 comparators at 6 h for both intravenous and intramuscular administration (Figure 2; n = 3 per group). The mRNA cargo and endpoint differ from the CAR-T assay.
Primary publication Gao H, et al. iScience 29 (2026), 117390. doi:10.1016/j.isci.2026.117390. Lipid identity: SI Methods S1, amine core 6, lipid structure table (SI pp. 2, 5, 8); LNP physical data: SI Table S1; B13: SI Table S2; results: main Figs. 2–7.