Literature LNP formulation
Components / mol% | PA97MD101 / DSPC / Cholesterol / PEG-DMG (PEG2000) = 50 / 10 / 38.5 / 1.5 (total 100%)
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PA97MD101
Literature records
Search the tables and observations reproduced from the product literature guide. Rows may represent measurements, recipes or experimental context; they are not all independent experiments.
Literature LNP formulation
N/P ratio | Not reported
Literature LNP formulation
Preparation | Microfluidic mixing; ethanol 3 mL/min + aqueous phase 9 mL/min; total flow 12 mL/min
Literature LNP formulation
Aqueous phase | 10 mM sodium citrate, pH 4.5
Literature LNP formulation
Post-processing | At least three 30 kDa centrifugal-filter wash cycles, then 0.22 μm filtration; adjusted to 1 mg/mL total lipid; formulated LNPs stored at 4°C until use
Literature LNP formulation
Apparent LNP pKa | 6.85 (TNS fluorescence titration)
Literature LNP formulation
mRNA encapsulation | 91.19 ± 0.58% SD; n = 3 independent samples (RiboGreen assay)
Literature LNP formulation
Mass ratio | 40:1 lipid:mRNA is specified for the in vitro screen; it is not an N/P ratio and is not assigned here to the in vivo experiments
Hepatic luciferase expression
Cargo | Fluc mRNA
Hepatic luciferase expression
Route | Intravenous, tail vein
Hepatic luciferase expression
Dose | 0.1 mg/kg mRNA, single injection
Hepatic luciferase expression
Model | C57BL/6 mice, 6-8 weeks; n=3/group
Hepatic luciferase expression
Readout time | 6 h
Cre-dependent liver recombination
Cargo | Cre mRNA
Cre-dependent liver recombination
Route | Intravenous, tail vein
Cre-dependent liver recombination
Dose | 0.25 mg/kg mRNA, single injection
Cre-dependent liver recombination
Model | mTmG reporter mice; n=3/group
Cre-dependent liver recombination
Readout time | Day 3
Cas9/sgTtr delivery and TTR suppression
Cargo | Cas9 mRNA + sgTtr, 5:1 mass ratio
Cas9/sgTtr delivery and TTR suppression
Route | Intravenous, tail vein
Cas9/sgTtr delivery and TTR suppression
Dose | Per injection: Cas9 mRNA 0.25 mg/kg plus sgRNA 0.05 mg/kg; two consecutive daily injections. sgRNA dose and 0.30 mg/kg total RNA per injection are calculated from 5:1.
Cas9/sgTtr delivery and TTR suppression
Model | C57BL/6 mice; n=3/group
Cas9/sgTtr delivery and TTR suppression
Readout time | Day 7 after first injection
Secreted hEPO expression
Cargo | hEPO mRNA
Secreted hEPO expression
Route | Intravenous, tail vein
Secreted hEPO expression
Dose | 0.25 mg/kg mRNA
Secreted hEPO expression
Model | C57BL/6 mice; n=3/group
Secreted hEPO expression
Readout time | 24 h