lipid 14
Instructions for Use
Product information
LIPID 14 is a novel ionizable lipid used for mRNA delivery.In 2021, Elia et al. used lipid 2 LNPs and lipid 14 LNPs to deliver mRNA encoding SARSCoV-2 human Fc-conjugated receptor binding domain (RBDhFc mRNA). While both lipid 274 LNP RBD-hFc mRNA and lipid 14 LNP RBD-hFc mRNA induced equal cellular and humoral responses in mice at an mRNA dose of 5 μg, only lipid 14 LNP RBD-hFc mRNA exhibited strong immunogenicity following intradermal administration. Both intradermal administration and intramuscular administration of lipid 14 LNPs could activate antigen presenting cells (APCs), thus inducing cellular responses.
| CAS No. | Not available |
| Chemical Name | lipid 14 |
| Formula | C56H110N2O6 |
| M.Wt | 907.48 |
| Purity | >95% |
| Storage | Original product: up to 1 year under the storage conditions stated on the vial, kept tightly sealed. Stock solutions: aliquots at -20°C, generally up to one month. |
| Shipping condition | Ships from Shanghai. Pure lipid is stable during ice-pack transport. |
Formulation & study context
Literature information refers to the cited study and its particular formulation. Administration routes are research context, not clinical instructions or a guarantee of performance.
A complete product-specific formulation has not been verified. No standard ratio is substituted.
| N/P ratio | Not reported in the verified record |
| Cargo | Not available in the verified record |
| Administration route | Not available in the verified record |
| Animal model | Not available in the verified record |
| Formulation process | Not available in the verified record |
N/P is the molar ratio of specified lipid nitrogen groups to nucleic-acid phosphate groups. A lipid:cargo mass ratio is a different quantity and must not be used as N/P.
Stock-solution preparation table
Calculated from the product-page molecular weight: 907.48 g/mol. Volumes are final solution volumes.
| Lipid mass | 1 mM | 5 mM | 10 mM |
|---|---|---|---|
| 10 mg | 11.020 mL | 2.204 mL | 1.102 mL |
| 25 mg | 27.549 mL | 5.510 mL | 2.755 mL |
| 50 mg | 55.098 mL | 11.020 mL | 5.510 mL |
| 100 mg | 110.195 mL | 22.039 mL | 11.020 mL |
| 250 mg | 275.488 mL | 55.098 mL | 27.549 mL |
Volume (mL) = mass (mg) × 1000 / [MW (g/mol) × concentration (mM)]. These are mathematical conversions, not measured solubility or validated solvent recommendations. Confirm solvent compatibility and the molecular weight of the exact material before use.
Open editable solution calculators →Handling & storage
Provided that storage conditions are as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 1 year.
Wherever possible, prepare and use solutions on the same day. If stock solutions must be prepared in advance, store them as aliquots in tightly sealed vials at -20°C. Generally, these will be usable for up to one month.
Before use, and prior to opening the vial, allow the product to equilibrate to room temperature for at least 1 hour.
References
1 An Effective mRNA-LNP Vaccine Against the Lethal Plague Bacterium Edo Kon,Dan Peer, Ofer Cohen-doi: biorxiv, https://doi.org/10.1101/2022.08.07.503096 ||2 Elia, U.; Ramishetti, S.; Rosenfeld, R.; Dammes, N.; Bar-Haim, E.; Naidu, G. S.; Makdasi, E.; Yahalom-Ronen, Y.; Tamir, H.; Paran, N.;et al. Design of SARS-CoV-2 hFc-Conjugated Receptor-Binding Domain mRNA Vaccine Delivered via Lipid Nanoparticles. ACS Nano 2021 ||3 : A single-dose F1-based mRNA-LNP vaccine provides protection against the lethal plague bacterium E Kon, Y Levy, U Elia, H Cohen, I Hazan-Halevy… - Science Advances, 2023