Lipid CS22021
Instructions for Use
Product information
CS22021 is an ionizable sterol lipid studied in three-component LNPs containing a helper phospholipid and M-DMG-PEG2K, without added free cholesterol. The supporting information reports formulation ratios, N/P and particle properties. The published abstract reports localized expression after intramuscular administration and enhanced CD8+ T-cell responses in a preclinical VZV mRNA vaccine study.
| CAS No. | Not available |
| Chemical Name | Lipid CS22021 |
| Formula | C58H101O5N3 |
| M.Wt | 920.46 |
| Purity | >98% |
| Storage | Original product: up to 1 year under the storage conditions stated on the vial, kept tightly sealed. Stock solutions: aliquots at -20°C, generally up to one month. |
| Shipping condition | Ships from Shanghai. Pure lipid is stable during ice-pack transport. |
Formulation & study context
Literature information refers to the cited study and its particular formulation. Administration routes are research context, not clinical instructions or a guarantee of performance.
| Component | Reported component ratio | Role |
|---|---|---|
| CS22021 | 49.25 | Ionizable lipid |
| DOPE | 49.25 | Helper phospholipid |
| M-DMG-PEG2K | 1.5 | PEG lipid |
| Reported ratio sum | 100 | Original ratio retained |
| N/P ratio | 8 |
| Cargo | Luciferase mRNA reporter; gE vaccine cargo is listed separately in Table S2. |
| Administration route | IV reporter assay (SI Fig. S5); IM localization (abstract only). |
| Dose | IV: 5 micrograms mRNA/mouse. IM dose not available in supplied SI/abstract. |
| Animal model | IV: male BALB/c mice, 6-8 weeks, n=3. |
| Formulation process | Not specified in supplied SI or abstract. |
N/P is the molar ratio of specified lipid nitrogen groups to nucleic-acid phosphate groups. A lipid:cargo mass ratio is a different quantity and must not be used as N/P.
Stock-solution preparation table
Calculated from the product-page molecular weight: 920.46 g/mol. Volumes are final solution volumes.
| Lipid mass | 1 mM | 5 mM | 10 mM |
|---|---|---|---|
| 10 mg | 10.864 mL | 2.173 mL | 1.086 mL |
| 25 mg | 27.160 mL | 5.432 mL | 2.716 mL |
| 50 mg | 54.321 mL | 10.864 mL | 5.432 mL |
| 100 mg | 108.641 mL | 21.728 mL | 10.864 mL |
| 250 mg | 271.603 mL | 54.321 mL | 27.160 mL |
Volume (mL) = mass (mg) × 1000 / [MW (g/mol) × concentration (mM)]. These are mathematical conversions, not measured solubility or validated solvent recommendations. Confirm solvent compatibility and the molecular weight of the exact material before use.
Open editable solution calculators →Handling & storage
Provided that storage conditions are as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 1 year.
Wherever possible, prepare and use solutions on the same day. If stock solutions must be prepared in advance, store them as aliquots in tightly sealed vials at -20°C. Generally, these will be usable for up to one month.
Before use, and prior to opening the vial, allow the product to equilibrate to room temperature for at least 1 hour.
References
Cited literature for this formulation and performance dataset Wang Z, Yan Z, Yan S, et al. Ionizable Sterol Lipid-Based Three-Component Lipid Nanoparticles for Localized Delivery of mRNA Vaccine with Stronger Cellular Immune Responses. ACS Applied Materials & Interfaces. 2025;17(25):36377-36386. DOI: 10.1021/acsami.5c04597 Numerical data: supplied 16-page SI, Tables S1-S2 and Figs. S5-S6. IM localization and immune-response claims: indexed abstract only. Full article methods were not accessible. Publisher Supporting Information · PubMed abstract