TTP-3
Instructions for Use
Product information
TTP-3 is an asymmetric Ugi-derived ionizable lipid identified from a 1,000-member library designed for pulmonary delivery of structured suppressor tRNA. In the cited study, TTP-3 was selected in a cystic-fibrosis-relevant air–liquid interface model containing artificial mucus, then evaluated in optimized LNP formulations for intratracheal delivery. All formulation and performance data below are literature-reported and remain specific to the stated cargo, preparation process, dose, model and administration route.
| CAS No. | Not assigned |
| Chemical Name | TTP-3 |
| Formula | C 51 H 97 N 3 O 4 (calculated from the literature-reported structure) |
| M.Wt | 816.35 g/mol (calculated) |
| Purity | >98% |
| Storage | Original product: up to 1 year under the storage conditions stated on the vial, kept tightly sealed. Stock solutions: aliquots at -20°C, generally up to one month. |
| Shipping condition | Pure lipid, shipped from Shanghai with ice packs. |
Formulation & study context
Literature information refers to the cited study and its particular formulation. Administration routes are research context, not clinical instructions or a guarantee of performance.
| Component | mol% | Role |
|---|---|---|
| TTP-3 | 60.0 | Ionizable lipid |
| DOPE | 10.0 | Helper phospholipid |
| β-Sitosterol | 27.5 | Sterol lipid |
| C14-PEG2000 | 2.5 | PEG lipid |
| Total | 100.0 |
| N/P ratio | Not reported in the cited study |
| Lipid:cargo mass ratio | 13.5:1 |
| Cargo | Suppressor tRNA |
| Administration route | Intratracheal administration |
| Animal model | LumA (FLuc R387X) mouse |
| In vivo study dose | 1.25 mg/kg · day 3 readout |
| Cell-tracking regimen | 3 doses × 1 mg/kg |
N/P is the molar ratio of specified lipid nitrogen groups to nucleic-acid phosphate groups. A lipid:cargo mass ratio is a different quantity and must not be used as N/P.
Stock-solution preparation table
Calculated from the product-page molecular weight: not available g/mol. Volumes are final solution volumes.
| Lipid mass | 1 mM | 5 mM | 10 mM |
|---|---|---|---|
| 10 mg | MW unavailable | MW unavailable | MW unavailable |
| 25 mg | MW unavailable | MW unavailable | MW unavailable |
| 50 mg | MW unavailable | MW unavailable | MW unavailable |
| 100 mg | MW unavailable | MW unavailable | MW unavailable |
| 250 mg | MW unavailable | MW unavailable | MW unavailable |
Volume (mL) = mass (mg) × 1000 / [MW (g/mol) × concentration (mM)]. These are mathematical conversions, not measured solubility or validated solvent recommendations. Confirm solvent compatibility and the molecular weight of the exact material before use.
Open editable solution calculators →Handling & storage
Provided that storage conditions are as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 1 year.
Wherever possible, prepare and use solutions on the same day. If stock solutions must be prepared in advance, store them as aliquots in tightly sealed vials at -20°C. Generally, these will be usable for up to one month.
Before use, and prior to opening the vial, allow the product to equilibrate to room temperature for at least 1 hour.
References
Cited literature for this formulation and performance dataset Chen J, Zhou M, Dong S, et al. Nonviral delivery of chemically modified tRNA rescues nonsense mutations in cystic fibrosis. Science . 2026;393:eaeb0054. DOI: 10.1126/science.aeb0054 Formulation: main text and Methods; performance: Fig. 3K, Fig. 4C–F, and supplementary Figs. S13–S14 and S18.