DC49907 · Updated 28 October 2026
5A2-SC8
Instructions for Use
Before use
Check the supplied lot number, batch COA, approved SDS, quantity and retest date. Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
For small quantities of viscous or oily lipid, volumetric transfer can reduce weighing losses. Confirm solvent compatibility and solubility before preparing a stock; calculated volumes do not establish solubility.
Stock-solution planning
Volume (mL) = mass (mg) × 1000 / [molecular weight × concentration (mM)].
| Mass | 10 mM | 20 mM |
|---|---|---|
| 10 mg | 0.5430 mL | 0.2715 mL |
| 25 mg | 1.3574 mL | 0.6787 mL |
| 50 mg | 2.7148 mL | 1.3574 mL |
| 100 mg | 5.4296 mL | 2.7148 mL |
Literature formulation reference
| Component | DC Chemicals product | Molecular weight | Molar ratio | Mass at 1 µmol total lipid (mg) |
|---|---|---|---|---|
| 5A2-SC8 | DC49907 | 1841.76 | 15 | 0.438514 |
| DOPE | DC33634 | 744.03 | 15 | 0.177150 |
| Cholesterol | DCD-041 | 386.65 | 30 | 0.184119 |
| DMG-PEG2000 | DC40169 | 2507 | 3 | 0.119381 |
2024 base formulation: 5A2-SC8/DOPE/cholesterol/DMG-PEG2000 = 15:15:30:3 relative molar parts (sum 63). The paper also presents rounded percentages 23.8:23.8:47.6:4.8. Total lipid:RNA weight ratios vary by experiment: 40:1 for luciferase/characterization, 20:1 for Cre and 10:1 for the reported genome-editing cargos. None is an N/P ratio. This composition is not assigned to the 2016 siRNA experiments.
Read the complete study conditions, biological results, tolerability and figures →
References
- Zhou K, et al. Modular degradable dendrimers enable small RNAs to extend survival in an aggressive liver cancer model. PNAS (2016). DOI: 10.1073/pnas.1520756113.
- Lian X, et al. Bone-marrow-homing lipid nanoparticles for genome editing in diseased and malignant haematopoietic stem cells. Nature Nanotechnology (2024). DOI: 10.1038/s41565-024-01680-8.