Table 1
Component: BP-200-C12 | Molecular weight: 2374.6 | Reported molar entry: 35 | Calculated mass: Not calculated
sales@dcchemicals.com
BP-200-C12
Literature records
Search the tables and observations reproduced from the product literature guide. Rows may represent measurements, recipes or experimental context; they are not all independent experiments.
Table 1
Component: DOPE | Molecular weight: 744.03 | Reported molar entry: 16 | Calculated mass: Not calculated
Table 1
Component: Cholesterol | Molecular weight: 386.65 | Reported molar entry: 46.5 | Calculated mass: Not calculated
Table 1
Component: 14:0 PEG2000 PE | Molecular weight: Not confirmed | Reported molar entry: 2.5 | Calculated mass: Not calculated
Table 2
Parameter: LNP formation | Reported procedure / limitation: Ethanolic lipid phase mixed with citrate-buffered aqueous RNA by vigorous pipetting; dialysis against 1× PBS for 2 h; 0.22 µm filtration. | Source: Methods; exact buffer pH, phase ratio and mixing rate not recovered
Table 2
Parameter: N/P / mass ratio | Reported procedure / limitation: N/P and a directly verified lipid:RNA mass ratio are not established here. | Source: No conversion from particle size or loading is attempted
Table 2
Parameter: Reported lipid batch | Reported procedure / limitation: BP-200-C12 2.20 × 10⁻⁷ mol; DOPE 1.01 × 10⁻⁷; cholesterol 2.92 × 10⁻⁷; PEG lipid 1.57 × 10⁻⁸. | Source: Main Methods; rounded values
Table 2
Parameter: HA-disc workflow | Reported procedure / limitation: 10 × 2 mm HA discs; overnight preconditioning in DMEM + 0.2% collagen; incubate with 150 µL LNP containing 2,000 ng RNA at 37 °C with shaking for 24 h; wash; seed 50,000 BJ cells or 100,000 mMSCs. | Source: Methods; assay readout 48 h after seeding
Table 2
Parameter: Bone-graft experiment | Reported procedure / limitation: 4 million tdROSA mMSCs, 40 mg HA/TCP and 20 µL LNP at 520 ng RNA/µL per implant; subcutaneous implantation in nude mice. | Source: Methods; RNA per implant = 10.4 µg, calculated
Table 3
Measurement / batch: siMiTEX (siRNA formulation) | Reported result: 154.3 nm; PDI 0.203; zeta potential −29.28 mV | Evidence context: SI Fig. S13; not transferred to the mRNA-loaded batch
Table 3
Measurement / batch: PEG-lipid identity | Reported result: 14:0 PEG2000 PE is a phospholipid-linked PEG component | Evidence context: Methods; not DMG-PEG2000
Table 3
Measurement / batch: Complete mass conversion | Reported result: Withheld until the exact PEG material molecular-weight basis is confirmed | Evidence context: DOPE and cholesterol values alone do not define the full mass recipe
Table 4
Experiment and dose: Free LNP at low RNA dose mMSCs; Cre mRNA; 20 ng / 20,000 cells | Readout and comparator: Readout: SI assay endpoint Comparator: C12-200 LNP | Result and source: MiTEX 22.9% versus C12-200 57.5% reporter-positive cells: MiTEX is inferior in this free-particle condition. SI Fig. S4
Table 4
Experiment and dose: Free LNP at higher dose mMSCs; Cre mRNA; 200 ng / 20,000 cells | Readout and comparator: Readout: SI assay endpoint Comparator: C12-200 LNP | Result and source: 71.2% versus 70.3%; no significant difference reported. SI Fig. S4
Table 4
Experiment and dose: Mineral binding HA binding assay; fluorescent-lipid tracking | Readout and comparator: Readout: 120 min Comparator: C12-200 LNP | Result and source: Approximately 80% versus 10% bound at 120 min. This kinetic assay is distinct from the 24 h disc-loading workflow. Fig. 2 / mineral-binding study
Table 4
Experiment and dose: After-wash HA retention HA discs; 2,000 ng mRNA input | Readout and comparator: Readout: After loading and washing Comparator: C12-200 LNP | Result and source: Approximately 200 ng retained with MiTEX versus <50 ng with control LNPs. Input dose and retained dose are different quantities. Fig. 3b
Table 4
Experiment and dose: Mineral-mediated Cre delivery mMSCs seeded on loaded HA discs; Cre reporter | Readout and comparator: Readout: 48 h after seeding Comparator: C12-200 LNP on HA | Result and source: Approximately 80% versus 17.92% reporter-positive cells. The advantage appears in the mineral-bound delivery context. Fig. 3e–f
Table 4
Experiment and dose: STAT3 silencing Gingival fibroblasts; STAT3 siRNA, 1–100 nM | Readout and comparator: Readout: 24 h Comparator: Control formulation and untreated cells | Result and source: >90% knockdown at 50 and 100 nM; both tested carrier systems exceed 90% at 50 nM. No superiority is inferred from this endpoint alone. siRNA results; SI characterization
Table 4
Experiment and dose: Bone-graft reporter persistence Subcutaneous HA/TCP/mMSC implant with Cre-mRNA LNP | Readout and comparator: Readout: Results describe 60 days; Methods describe 8 weeks Comparator: Control-graft conditions | Result and source: Reporter evidence is evaluated in an implanted scaffold/cell system, not as direct systemic bone targeting. The duration wording is retained as a source discrepancy. Bone-graft Results and Methods