Table 2
Parameter: Formulation identity | Reported procedure / limitation: TMF30 contains compound 43; it is not a generic formulation for every lipid in the patent. | Source: Table 3
sales@dcchemicals.com
Lipid 43
Literature records
Search the tables and observations reproduced from the product literature guide. Rows may represent measurements, recipes or experimental context; they are not all independent experiments.
Table 2
Parameter: Preparation cross-reference | Reported procedure / limitation: The patent states TMF17–TMF53 follow the TMF2 preparation with the Table 3 component substitutions. Exact TMF2 mixing/buffer parameters are not reproduced in this revision. | Source: Paragraph [0511], printed p.129
Table 2
Parameter: RNA ratio / N/P | Reported procedure / limitation: Not transcribed from a verified preparation passage; no inferred N/P is supplied. | Source: Preparation cross-reference requires completion
Table 2
Parameter: Animal reporter assay | Reported procedure / limitation: Female ICR mice, 6–8 weeks; retro-orbital intravenous administration, 0.5 mg/kg RNA; luciferin 150 mg/kg before imaging. | Source: Paragraph [0517], printed p.132
Table 3
Measurement / batch: TMF30 hydrodynamic size | Reported result: 210.60 ± 1.83 nm | Evidence context: Table 4, printed p.132; replicate/statistical basis not specified in the transcribed table
Table 3
Measurement / batch: TMF30 PDI | Reported result: 0.136 ± 0.018 | Evidence context: Table 4
Table 3
Measurement / batch: Encapsulation / apparent pKa | Reported result: Not established in the reviewed TMF30 table | Evidence context: No value inferred
Table 4
Experiment and dose: Spleen reporter expression ICR mice; luciferase RNA; intravenous 0.5 mg/kg | Readout and comparator: Readout: 4 h after dosing Comparator: TMF17, DODAP-based formulation with the same listed helper ratios | Result and source: TMF30 spleen flux 1.31 × 10⁹ p/s; TMF17 1.45 × 10⁶ p/s. These are tissue expression signals, not percentages of injected particles. Table 5, printed p.134; n/statistical test not stated in the transcribed table
Table 4
Experiment and dose: Liver reporter expression Same TMF30 reporter experiment | Readout and comparator: Readout: 4 h Comparator: TMF17 liver flux 1.69 × 10⁶ p/s | Result and source: TMF30 liver flux 3.56 × 10⁷ p/s. Higher spleen signal does not imply absence of liver delivery. Table 5; paragraph [0517]
Table 4
Experiment and dose: Spleen:liver selectivity Same assay; compare individual formulations | Readout and comparator: Readout: 4 h Comparator: TMF29 (compound 38): spleen 5.73 × 10⁸, liver 8.48 × 10⁶ p/s; reported ratio 69.5 | Result and source: TMF30 reported ratio is 38.8; dividing its displayed mean fluxes gives 36.80 (calculated). TMF30 has greater absolute spleen flux than TMF29 but a lower reported spleen:liver ratio. Table 5; calculated ratio is identified separately