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DCL0001 · Updated 24 October 2026

Antioxidant lipid AO16
Formulation & Performance

This edition organizes the previously reviewed, source-linked product record. It is not a new independent review of every paper or supporting file. Reported and calculated values retain their original labels.

Literature-reported formulation composition

The paper reports AO16 as an additive relative to SM-102. The values below retain the reported ratios; normalized mol% is shown separately.

ComponentReported molar ratioNormalized mol%Role
AO162.502.44Ionizable lipid
SM-10250.0048.78Ionizable lipid
DSPC10.009.76Helper phospholipid
Cholesterol38.5037.56Sterol
DMG-PEG20001.501.46PEG lipid
Reported ratio sum102.50100.00AO16 addition relative to SM-102
Not reported in the cited article
20:1 (total lipid:mRNA); this is not an N/P ratio.
Citrate buffer pH 4.0 and ethanol phase at 3:1 aqueous:ethanol, vortexed 5 min, incubated 10 min, then dialyzed against PBS for at least 12 h.
HEK293T reporter assay and reactive-species challenge; AO16-specific data are in vitro screening data.
mRNA screening used 250 ng per well; oxidative challenge used 0.05–0.50 mM reactive species for 2 h before dialysis.

AO16 literature screening results

Literature-reported67% for AO16 LNPs versus 57% for the commercial control LNPReactive species panel: O2−, ·OH, H2O2, ClO− and ONOO−; 5 mol% AO16 relative to SM-102Supplementary Information p. 76, Supplementary Table 2.
Literature-reported32% for AO16 LNPs versus 37% for the commercial control LNPSame five-species panel at the higher challenge concentrationSupplementary Information p. 78, Supplementary Table 2.
Literature-reportedO2− 87; ·OH 42; H2O2 72; ClO− 69; ONOO− 64These are the source table values used to calculate the reported average score of 67.Supplementary Information p. 76, Supplementary Table 2.

Interpretation boundary

What the source supports

The cited article supports AO16 as an in vitro antioxidant-lipid screening candidate with the formulation and challenge conditions listed above.

Nature Communications 2026;17:7166; DOI 10.1038/s41467-026-73512-3.
What is not assigned to AO16

AO12 was the lead selected for the paper's later regeneration, biodistribution and gene-editing experiments. Those AO12-specific animal results are not presented as AO16 product performance.

Product-page evidence boundary.
Literature Data DisclaimerAll formulation parameters and performance values shown here are derived from the cited publication and supplementary information. They are reference data, not product release specifications or performance guarantees. DC Chemicals supplies the lipid compound only.
Cited literatureLi B, Zhao M, Yang B, et al. Antioxidant lipid nanoparticles enhance mRNA stability for regeneration therapy and gene editing. Nature Communications. 2026;17:7166. DOI: 10.1038/s41467-026-73512-3