Literature-reported formulation composition
Component: E20 | mol%: 35.9 | Role: Ionizable lipid
sales@dcchemicals.com
E20
Literature records
Search the tables and observations reproduced from the product literature guide. Rows may represent measurements, recipes or experimental context; they are not all independent experiments.
Literature-reported formulation composition
Component: DOPE | mol%: 24.2 | Role: Helper phospholipid
Literature-reported formulation composition
Component: Cholesterol | mol%: 38.7 | Role: Sterol
Literature-reported formulation composition
Component: DMG-PEG2000 | mol%: 1.2 | Role: PEG lipid
Literature-reported formulation composition
Component: Reported total | mol%: 100 | Role: Source values retained
Reported observation
Literature-reported Apparent SNP pKa 4.99 R18 fluorescence assay · pKa for becoming neutral Main article Figure 2b; SI Section 5.4
Reported observation
Literature-reported Hydrodynamic diameter 90-100 nm Post-formulation DLS characterization · Range reported for the five top SNPs E3, E7, E12, E15 and E20; not an E20-only exact value SI Figure S76 and text, p. 135
Reported observation
Literature-reported Polydispersity index <0.2 Post-formulation DLS characterization · Reported for the SNP formulation panel; not an E20-only exact value SI Figure S77 and text, p. 135
Reported observation
Literature-reported Blood hEPO concentration 600 mU/mL 24 h after 0.1 mg/kg IV hEPO mRNA · Reported as equivalent to MC3-DLin LNPs and 100-fold above the cited therapeutic threshold Main article Figure 3b and text
Reported observation
Literature-reported Inflammatory cytokine response No increase in IL-6, IL-1β or MIP-2 4 h after 1 mg/kg IV luciferase mRNA in LPS-pretreated mice · Traditional LNP comparators increased the measured cytokines in the LPS-pretreated model Main article Figure 3c-f; SI Figures S95-S97
Reported observation
Literature-reported pDNA study survival All E20-SNP-treated mice survived the 7-day observation period After 1 mg/kg IV luciferase nanoplasmid DNA · All MC3-DLin LNP-pDNA-treated mice died within 24 h Main article Figure 3g-i and text; SI Section 6.2
Reported observation
Human PBMC study E20-SNPs efficiently delivered luciferase mRNA to human PBMCs and did not induce any of the 14 cytokines evaluated in the reported experiment. Human PBMCs from four donors; 2,000 ng/mL mRNA; 24 h · Main article Figure 4; SI Figures S90-S93
Reported observation
Acute lung injury study E20-SNPs carrying IL-22 mRNA were reported to be more effective than the traditional LNP comparators in the LPS-induced acute-lung-injury model without exacerbating pre-existing inflammation. BALB/c mice; 3 mg/kg intratracheal LPS followed 3 h later by 2.5 mg/kg intratracheal LNP/IL-22 mRNA · Main article acute-lung-injury section; SI Section 6.4
Reported observation
Charge-switching behavior The study reports that E20 SNPs are neutral or negatively charged at physiological pH and become positively charged under acidic formulation/endosomal conditions. R18 apparent-pKa and pH-dependent zeta-potential experiments · Main article Figure 2b; SI Figures S78-S80