Literature-reported formulation composition
Component: AMG514 | mol%: 35 | Role: Ionizable lipid
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AMG514
Literature records
Search the tables and observations reproduced from the product literature guide. Rows may represent measurements, recipes or experimental context; they are not all independent experiments.
Literature-reported formulation composition
Component: DOPE | mol%: 16 | Role: Helper phospholipid
Literature-reported formulation composition
Component: Cholesterol | mol%: 46.5 | Role: Sterol
Literature-reported formulation composition
Component: DMG-PEG2k | mol%: 2.5 | Role: PEG lipid
Literature-reported formulation composition
Component: Reported total | mol%: 100 | Role: Source values retained
Reported observation
Literature-reported mRNA encapsulation efficiency 97% Post-formulation characterization · Representative cKK-E12 LNP: 56% Supplementary Table 2, internal p. 25 / PDF p. 26
Reported observation
Literature-reported Z-average diameter 102.7 nm Post-formulation characterization · Representative cKK-E12 LNP: 86.0 nm Supplementary Table 2, internal p. 25 / PDF p. 26
Reported observation
Literature-reported Polydispersity index 0.133 Post-formulation characterization · Representative cKK-E12 LNP: 0.155 Supplementary Table 2, internal p. 25 / PDF p. 26
Reported observation
Literature-reported Apparent LNP pKa approximately 7.5 Post-formulation characterization · Approximately 6.5 is described as characteristic of the cited liver-tropic formulations Main text p. 11; Supplementary Figure 21b
Reported observation
Literature-reported Splenic FLuc expression approximately four-fold higher 6 h after a single 1 microgram IV FLuc-mRNA dose · Versus cKK-E12 LNP; reduced liver signal was also reported Main text p. 9; Extended Data Figure 4c,d
Reported observation
Splenic antigen-presenting-cell transfection In the Ai14/Cre reporter experiment, the plotted fractions were approximately 67% versus 40% tdTomato-positive dendritic cells and approximately 60% versus 40% tdTomato-positive macrophages for AMG514 versus cKK-E12. Cre mRNA, IV, n=4-5; the figure caption reports 5 micrograms and 48 h, while Methods report 0.25 mg/kg and 72 h · Extended Data Figure 4e and Methods
Reported observation
Antigen-specific CD8 T-cell response AMG514-formulated NIK plus OVA generated approximately 15% OVA-tetramer-positive CD8 T cells after boosting, compared with approximately 6% for the cKK-E12 formulation. C57BL/6J mice; total 3 micrograms RNA; days 0 and 14; n=5; route not stated for this experiment · Main Figure 6f-h and text p. 11
Reported observation
B16-OVA tumor challenge AMG514 NIK plus OVA produced complete rejection in five of five mice, compared with three of five for cKK-E12 NIK plus OVA and one of five for AMG514 OVA alone. The authors state that the AMG514 advantage over cKK-E12 was not statistically significant. Preclinical prophylactic vaccine and tumor-challenge study · Main Figure 6i,j and text p. 11
Reported observation
Protein-corona profile The reported AMG514 corona was enriched in vitronectin, coagulation proteins and selected apolipoproteins. This association does not by itself prove a receptor-mediated targeting mechanism. The main text refers to plasma, while the Supplementary Figure 21 caption refers to serum · Main text p. 11 and Supplementary Figure 21