Tidal Lipid 40
Instructions for Use
Product information
Tidal Lipid 40is an ionizable cationic lipid engineered to deliver RNA with high precision to immune cells like macrophages. Based on Tidal Therapeutics' patent US 20250205169A1, Its pH-responsive design shifts from a +8 mV charge at pH 5.5 (enabling endosomal escape) to near-neutral at pH 7.4 (reducing off -target binding), ensuring efficient intracellular release while maintaining blood stability. In lipid nanoparticles, Lipid 40 achieves 65% transfection efficiency in human macrophages—surpassing benchmarks like ALC-0315—and protects >95% of RNA payloads from degradation. Critically, it maintains particle integrity after freeze-thaw cycles with minimal size drift (<5 nm) and excels in in vivo targeting, driving potent gene expression in tumor-associated macrophages while avoiding liver/spleen accumulation. This combination of precision delivery, stability, and low toxicity makes it ideal for immunotherapies, such as reprogramming M2 macrophages to anti-tumor M1 states.
| CAS No. | Not available |
| Chemical Name | Tidal Lipid 40 |
| Formula | C63H118O11N2 |
| M.Wt | 1,079.64 |
| Purity | >98% |
| Storage | Original product: up to 1 year under the storage conditions stated on the vial, kept tightly sealed. Stock solutions: aliquots at -20°C, generally up to one month. |
| Shipping condition | Ships from Shanghai. Pure lipid is stable during ice-pack transport. |
Formulation & study context
Literature information refers to the cited study and its particular formulation. Administration routes are research context, not clinical instructions or a guarantee of performance.
A complete product-specific formulation has not been verified. No standard ratio is substituted.
| N/P ratio | Not reported in the verified record |
| Cargo | Not available in the verified record |
| Administration route | Not available in the verified record |
| Animal model | Not available in the verified record |
| Formulation process | Not available in the verified record |
N/P is the molar ratio of specified lipid nitrogen groups to nucleic-acid phosphate groups. A lipid:cargo mass ratio is a different quantity and must not be used as N/P.
Stock-solution preparation table
Calculated from the product-page molecular weight: 1079.64 g/mol. Volumes are final solution volumes.
| Lipid mass | 1 mM | 5 mM | 10 mM |
|---|---|---|---|
| 10 mg | 9.262 mL | 1.852 mL | 0.926 mL |
| 25 mg | 23.156 mL | 4.631 mL | 2.316 mL |
| 50 mg | 46.312 mL | 9.262 mL | 4.631 mL |
| 100 mg | 92.623 mL | 18.525 mL | 9.262 mL |
| 250 mg | 231.559 mL | 46.312 mL | 23.156 mL |
Volume (mL) = mass (mg) × 1000 / [MW (g/mol) × concentration (mM)]. These are mathematical conversions, not measured solubility or validated solvent recommendations. Confirm solvent compatibility and the molecular weight of the exact material before use.
Open editable solution calculators →Handling & storage
Provided that storage conditions are as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 1 year.
Wherever possible, prepare and use solutions on the same day. If stock solutions must be prepared in advance, store them as aliquots in tightly sealed vials at -20°C. Generally, these will be usable for up to one month.
Before use, and prior to opening the vial, allow the product to equilibrate to room temperature for at least 1 hour.
References
Not available