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Lipid H7T4-4

Version 1.78cb712b · 2026-09-13

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CAS No.Not available
Chemical NameLipid H7T4-4
SynonymsLipid H7T4 4
SMILESCCCCCCCCCCCCC(O)CN1CCN(CC(O)CCCCCCCCCCCC)CCN(CC(O)CCCCCCCCCCCC)CCN(CC(O)CCCCCCCCCCCC)CC1
FormulaC64H132N4O4
M.Wt1,021.78
PurityELSD-HPLC>95%
StorageOriginal product: up to 1 year under the storage conditions stated on the vial, kept tightly sealed. Stock solutions: aliquots at -20°C, generally up to one month.
Shipping conditionShips from Shanghai. Pure lipid is stable during ice-pack transport.
PublicationTransition Temperature-Guided Design of Lipid Nanoparticles for Effective mRNA Delivery-ACS Applied Materials & InterfacesVol 17-Jeong Eun ShinEun-jeong WonJunchao XuJong Cheol LeeJeong Kyu Bang*Michael J. Mitchell*Hyunjoo Cha-Molstad*

H7T4-4 is an ionizable lipid designed for mRNA delivery via lipid nanoparticles (LNPs). It features a cyclic amine headgroup (derived from cyclen tetrahydrochloride) and four C14 hydrophobic alkyl tails, synthesized through a Michael addition reaction between cyclen and 1,2-epoxytetradecane. With a high transition temperature (Tm = 58.6°C) due to strong intermolecular interactions from its cyclic headgroup and multi-tail structure, H7T4-4 alone forms rigid aggregates incompatible with mRNA encapsulation. However, when blended with low-Tm helper lipids (e.g., DOPE, Tm = -16°C), the system’s overall Tm decreases, enabling stable LNP formation. Optimized formulations (20% H7T4-4, 41% DOPE, 38% cholesterol, 1% DMG-PEG) exhibit efficient mRNA encapsulation (>90%) and transfection. Structural analyses (SAXS, cryo-TEM) confirm monodisperse LNPs with lamellar/hexagonal phases. In vivo, H7T4-4 LNPs show tumor-targeted and intranasal mRNA delivery with reduced off-target accumulation compared to SM-102-based LNPs. This rational design highlights Tm-guided helper lipid selection to overcome rigidity challenges in ionizable lipids.

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