DC60545 · Updated 28 October 2026
200Oi10
Instructions for Use
Before use
Check the supplied lot number, batch COA, approved SDS, quantity and retest date. Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.
For small quantities of viscous or oily lipid, volumetric transfer can reduce weighing losses. Confirm solvent compatibility and solubility before preparing a stock; calculated volumes do not establish solubility.
Stock-solution planning
Volume (mL) = mass (mg) × 1000 / [molecular weight × concentration (mM)].
| Mass | 10 mM | 20 mM |
|---|---|---|
| 10 mg | 0.7831 mL | 0.3915 mL |
| 25 mg | 1.9577 mL | 0.9788 mL |
| 50 mg | 3.9154 mL | 1.9577 mL |
| 100 mg | 7.8308 mL | 3.9154 mL |
Literature formulation reference
| Component | DC Chemicals product | Molecular weight | Molar ratio | Mass at 1 µmol total lipid (mg) |
|---|---|---|---|---|
| 200Oi10 | DC60545 | 1277.01 | 35 | 0.446953 |
| DOPE | Not uniquely mapped | 744.03 | 16 | 0.119045 |
| Cholesterol | DCD-041 | 386.65 | 46.5 | 0.179792 |
| C14-PEG2000 (source label) | Not uniquely mapped | Not established | 2.5 | Not calculable from disclosed data |
This table is the IV/IP comparison composition. The separate helper-lipid comparison uses 35:40:22.5:2.5, with DOPE, PS or DOTAP. The source gives a 10:1 lipidoid:mRNA mass ratio. PEG-lipid exact identity/MW is not resolved from the source label, so DMG-PEG2000 is not substituted.
Read the complete study conditions, biological results, tolerability and figures →
References
- Melamed JR et al. Ionizable lipid nanoparticles deliver mRNA to pancreatic beta cells via macrophage-mediated gene transfer. Science Advances 9, eade1444 (2023). DOI: 10.1126/sciadv.ade1444.
- Complete supporting information: Figure S1 and Figures S3-S8; main Figures 1B and 2B define the target-specific evidence.