DC31000 · Updated 28 October 2026

LP-01

Instructions for Use

Before use

Check the supplied lot number, batch COA, approved SDS, quantity and retest date. Pure form: −20 °C, 1 year; In solvent: −80 °C, 6 months; −20 °C, 1 month. Sealed and protected from light.

For small quantities of viscous or oily lipid, volumetric transfer can reduce weighing losses. Confirm solvent compatibility and solubility before preparing a stock; calculated volumes do not establish solubility.

Stock-solution planning

Volume (mL) = mass (mg) × 1000 / [molecular weight × concentration (mM)].

Mass	10 mM	20 mM
10 mg	1.1733 mL	0.5867 mL
25 mg	2.9333 mL	1.4666 mL
50 mg	5.8665 mL	2.9333 mL
100 mg	11.7331 mL	5.8665 mL

Literature formulation reference

Component	DC Chemicals product	Molecular weight	Molar ratio	Mass at 1 µmol total lipid (mg)
LP-01	DC31000	852.292	45	0.383531
DSPC	DC57005	790.15	9	0.071113
Cholesterol	DCD-041	386.65	44	0.170126
DMG-PEG2000	DC40169	2507	2	0.050140

Experimental Procedures, LNP Formulation: LP01 / DSPC / cholesterol / PEG2k-DMG = 45:9:44:2 mol%. Reported N/P = 4.5; mRNA:sgRNA = 1:1 by mass. These are different quantities. DMG-PEG2000 uses MW 2507 for the calculated component masses.

Read the complete study conditions, biological results, tolerability and figures →

References

Finn JD, Smith AR, Patel MC et al. A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing. Cell Reports 22, 2227–2235 (2018). DOI: 10.1016/j.celrep.2018.02.014.