All LNP calculators / Formulate LNPs
N/P & ionizable lipid:RNA ratio
Calculate ionizable lipid requirements with an explicit nitrogen-site and RNA-phosphate counting basis.
Your inputs
Results
Enter your inputs and select Calculate.
Calculations run in your browser. Exports contain your inputs and results.
Calculation basis
n(P) = RNA mass / mass per mole of counted phosphate. N/P = n(lipid) × counted N sites / n(P). The mass ratio is ionizable lipid:RNA only; DSPC, cholesterol and PEG-lipid are excluded. Stoichiometric N/P does not specify protonation or binding.