Datasheet — Lipid-392

DCL0020 · Updated 20 October 2026

Description:

Lipid-392 is a hydrazide-linked ionizable lipid with three tertiary amines, three palmitoyl-containing branches and a hydroxyl-bearing C10 arm. It is the carrier lipid in an optimized, cholesterol-free simplified targeted lipid nanoparticle (stLNP) containing Lipid-392, POPE and DMG-PEG2000 at 90:8.5:1.5 mol%. In mice, this formulation produced 4.93-fold greater lung luciferase expression than DOTAP-5-LNP at 0.25 mg/kg intravenous mRNA dosing, with approximately 99% of the measured lung/liver/spleen expression signal in the lung. The study also demonstrated lung-cell Cre reporter activation, adenine base editing, siRNA-mediated gene silencing and IL-10 mRNA delivery in an acute lung injury model. These preclinical results support evaluation for pulmonary RNA delivery; activity depends on formulation, payload and experimental conditions.

Chemical Information

Parameter	Value
Catalog number	DCL0020
Chemical Name	Lipid-392
CAS No.	Not available
Synonyms	Lipid 392; Lipid392; NNCC14
Formula	C74H147N9O7
M.Wt	1275.05
SMILES	CCCCCCCCCCCCCCCC(=O)NNC(=O)CCN(CCCN(C)CCCN(CCC(=O)NNC(=O)CCCCCCCCCCCCCCC)CC(O)CCCCCCCC)CCC(=O)NNC(=O)CCCCCCCCCCCCCCC
Purity	>95% by ELSD-HPLC
Storage	Follow the supplied batch label; protect from light and moisture. Compound-specific stock-solution shelf life is not established.
Publication	Fei Y, Lin Y, Liu P, Zhong K, Gao Y, Ma Q, Zhou Z, Luo S, Hu R, Cheng Q. Optimization of Lipid Nanoparticles for Safe and Versatile Lung-Targeted RNA Delivery and Disease Therapy. ACS Nano. 2026;20:15347–15362. doi:10.1021/acsnano.6c03013. DOI: 10.1021/acsnano.6c03013

Handling Guide

Follow the supplied batch label for storage. Protect the material from light and moisture; allow a sealed vial to equilibrate before opening. Prepare small aliquots and avoid repeated freeze–thaw cycles. Ethanol is used in the published lipid phase; a quantitative Lipid-392 solubility limit and stock-solution shelf life are not established.

Lipid-392 neat-material and stock-solution shelf life are not established by the cited paper. Follow the supplied batch label.

Formulation & Performance:

The selected Lipid-392 / POPE / DMG-PEG2000 formulation is 90:8.5:1.5 mol%, with N/P 10 in 20 mM acetate buffer at pH 4.0. RNA aqueous:ethanol is 3:1 v/v. This cholesterol-free stLNP was studied for intravenous RNA delivery in mice. Detailed component masses, dose-specific results and source discrepancies are provided in the combined guide.

Read the component mass table, preparation, experiment-specific results and references →

References:

Fei Y, Lin Y, Liu P, Zhong K, Gao Y, Ma Q, Zhou Z, Luo S, Hu R, Cheng Q. Optimization of Lipid Nanoparticles for Safe and Versatile Lung-Targeted RNA Delivery and Disease Therapy. ACS Nano. 2026;20:15347–15362. doi:10.1021/acsnano.6c03013. DOI: 10.1021/acsnano.6c03013