iChol15-C4A2
Datasheet

Product identity and specifications

Product description

Product overview Description & Application iChol15-C4A2 is an ionizable cholesteryl lipid that integrates a cholesterol moiety with an ionizable headgroup, enabling three-component lipid nanoparticles (Tc-LNPs) with DSPC and DMG-PEG2000. The published study links reduced ApoE adsorption and reduced dependence on ApoE/LDLR-mediated uptake to lower hepatic preference. In mice given luciferase mRNA intravenously at 0.1 mg/kg, the optimized formulation produced a spleen-to-liver expression ratio of 4.78 at 6 hours, versus 0.12 for the study's ALC-0315 control. This approximately 40-fold difference refers to the spleen-to-liver ratio, not absolute splenic expression. The study also reports high mRNA encapsulation, storage testing of loaded LNPs, and short-term tolerability under the tested conditions. RNA Delivery Spleen delivery Extrahepatic delivery RNA delivery research Cationic and ionizable lipids General research products

Handling guidance

Handling guidance Shipping, storage and sampling How is the lipid shipped, and how should I store it? We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. In solvent: store at −80 °C for up to 6 months or at −20 °C for up to 1 month. Keep sealed and protected from light. Avoid repeated thaw cycles for best results. Pure lipid or solution — which should I choose? Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed. How should I measure a small amount of oily or viscous lipid? Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first. Can you support formulation optimization and scale-up? Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows.

Formulation and performance

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Ordering

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References

• DOI: 10.1021/jacs.5c14870