113-AA-C8C14
Instructions for Use
Product information
113-AA-C8C14 is a β-hydroxy thioether-derived ionizable lipid identified through a two-stage lipid-library screen. In the cited study, its optimized LNP formulation preferentially delivered mRNA to the spleen after intravenous administration and was evaluated for transient in vivo generation of CD19-targeting CAR T cells. All formulation and performance data below are literature-reported and remain specific to the stated cargo, preparation process, dose, animal model and administration route.
| CAS No. | Not assigned |
| Chemical Name | 113-AA-C8C14 |
| Formula | C 61 H 117 N 5 O 8 S 2 |
| M.Wt | 1112.768 g/mol |
| Purity | 98% |
| Storage | Original product: up to 1 year under the storage conditions stated on the vial, kept tightly sealed. Stock solutions: aliquots at -20°C, generally up to one month. |
| Shipping condition | Pure lipid, shipped from Shanghai with ice packs. |
Formulation & study context
Literature information refers to the cited study and its particular formulation. Administration routes are research context, not clinical instructions or a guarantee of performance.
| Component | Molar ratio | Role |
|---|---|---|
| 113-AA-C8C14 | 35 | Ionizable lipid |
| DOPE | 16 | Helper phospholipid |
| Cholesterol | 46.5 | Sterol lipid |
| DMG-PEG2000 | 0.5 | PEG lipid |
| N/P ratio | Not reported in the cited study |
| Cargo | Luciferase mRNA / CD19-CAR mRNA |
| Administration route | Intravenous, tail vein |
| Screening model | Female C57BL/6 mice |
| Luciferase study | 0.1 mg/kg · 6 h readout |
| CAR-T efficacy model | CD19-Panc02 subcutaneous tumor |
| CAR mRNA regimen | 0.5 mg/kg (10 µg), 4 i.v. doses |
N/P is the molar ratio of specified lipid nitrogen groups to nucleic-acid phosphate groups. A lipid:cargo mass ratio is a different quantity and must not be used as N/P.
Stock-solution preparation table
Calculated from the product-page molecular weight: 1112.768 g/mol. Volumes are final solution volumes.
| Lipid mass | 1 mM | 5 mM | 10 mM |
|---|---|---|---|
| 10 mg | 8.987 mL | 1.797 mL | 0.899 mL |
| 25 mg | 22.466 mL | 4.493 mL | 2.247 mL |
| 50 mg | 44.933 mL | 8.987 mL | 4.493 mL |
| 100 mg | 89.866 mL | 17.973 mL | 8.987 mL |
| 250 mg | 224.665 mL | 44.933 mL | 22.466 mL |
Volume (mL) = mass (mg) × 1000 / [MW (g/mol) × concentration (mM)]. These are mathematical conversions, not measured solubility or validated solvent recommendations. Confirm solvent compatibility and the molecular weight of the exact material before use.
Open editable solution calculators →Handling & storage
Provided that storage conditions are as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 1 year.
Wherever possible, prepare and use solutions on the same day. If stock solutions must be prepared in advance, store them as aliquots in tightly sealed vials at -20°C. Generally, these will be usable for up to one month.
Before use, and prior to opening the vial, allow the product to equilibrate to room temperature for at least 1 hour.
References
Cited literature for this formulation and performance dataset Guo Q, Zhang Y, Yang X, et al. β-Hydroxy Thioether-Derived Ionizable Lipids for Spleen-Tropic mRNA Delivery and In Vivo Chimeric Antigen Receptor T Cell Engineering. ACS Nano . 2026;20:14602–14614. DOI: 10.1021/acsnano.6c00706 Formulation: main text, Experimental Section and Supporting Figs. S11–S12. Physicochemical data: Supporting Fig. S14. In vivo performance: main Fig. 4 and Supporting Figs. S13 and S23–S36.