Table 3
Component: 503O8,12 | Molecular weight: 1598.69 | Reported molar entry: 35 | Calculated mass: Not calculated
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503O8,12
Literature records
Search the tables and observations reproduced from the product literature guide. Rows may represent measurements, recipes or experimental context; they are not all independent experiments.
Table 3
Component: DOTAP (study salt basis unresolved) | Molecular weight: Not confirmed | Reported molar entry: 40 | Calculated mass: Not calculated
Table 3
Component: Cholesterol | Molecular weight: 386.65 | Reported molar entry: 22.5 | Calculated mass: Not calculated
Table 3
Component: 14:0 PEG2000 PE | Molecular weight: 2693.32 | Reported molar entry: 2.5 | Calculated mass: Not calculated
Table 4
Parameter: RNA / lipid relationship | Reported procedure / limitation: Ionizable lipidoid:mRNA 10:1 by mass; reported mRNA formulation concentration 0.09 mg/mL. N/P is not supplied. | Source: Experimental Section
Table 4
Parameter: Solvent and buffer | Reported procedure / limitation: Lipid phase ethanol:citrate 9:1 by volume; RNA in 10 mM citrate pH 4. | Source: Experimental Section
Table 4
Parameter: Mixing | Reported procedure / limitation: Equal volumes of lipid and RNA phases; vortex 3,200 rpm for 5 seconds. | Source: Experimental Section
Table 4
Parameter: Animal preparation | Reported procedure / limitation: PBS dialysis using 3.5 kDa MWCO membrane for 1 h. | Source: Experimental Section
Table 4
Parameter: Cargo | Reported procedure / limitation: 5-methoxyuridine-modified reporter mRNA; polyU used in the initial TNS library screen. | Source: Experimental Section; Results 2.2
Table 4
Parameter: Mouse reporter assay | Reported procedure / limitation: Female C57BL/6 mice, 6–8 weeks; 0.75 mg/kg mLuc by tail-vein injection. Luciferin at 3 h; tissue harvest approximately 15 min later. | Source: Experimental Section; Figs. 2–3
Table 4
Parameter: Cell reporter screen | Reported procedure / limitation: HeLa cells, 15,000 per well; mLuc 100 ng/well, 24 h; n=4. | Source: Experimental Section; Fig. 2c
Table 5
Measurement / batch: 16PE diameter / encapsulation / zeta | Reported result: Approximately 110 nm / 86% / −2.2 mV | Evidence context: Previously reviewed SI Fig. S1; visual graph estimates, n=4; no exact source-data values recovered
Table 5
Measurement / batch: 40PE diameter / encapsulation / zeta | Reported result: Approximately 100 nm / 88% / −4.8 mV | Evidence context: Previously reviewed SI Fig. S1; visual graph estimates, n=4
Table 5
Measurement / batch: 40TAP diameter / encapsulation / zeta | Reported result: Approximately 105 nm / 83% / −0.6 mV | Evidence context: Previously reviewed SI Fig. S1; visual graph estimates, n=4
Table 5
Measurement / batch: PDI / apparent pKa / N/P | Reported result: Not established for this product in the reviewed record | Evidence context: A TNS measurement at pH 5 does not determine pKa
Table 5
Measurement / batch: Particle sizing method | Reported result: Source method / caption discrepancy retained | Evidence context: Main methods describe nanoparticle tracking; SI caption describes a DLS number mean. Values are called mean particle size without resolving that inconsistency.
Table 6
Experiment and dose: Initial ionization screen PolyU-containing LNP library; TNS fluorescence at pH 5; n=12 | Readout and comparator: Readout: Plate-reader fluorescence Comparator: 580-member combinatorial library | Result and source: 503O8,12 proceeded into reporter delivery evaluation. A single-pH screen is neither a pKa curve nor proof of in vivo delivery. Fig. 2a–c
Table 6
Experiment and dose: Intravenous reporter potency 503O8,12 16PE; C57BL/6 mice; mLuc 0.75 mg/kg IV; n=3 | Readout and comparator: Readout: Approximately 3 h; excised-organ luminescence Comparator: Other screened ionizable lipidoids | Result and source: 503O8,12 generated measurable reporter expression, with total organ signal on the order of 10^8 photons/s by graph inspection. This is an estimate, not an exact mean. Fig. 2b; six-organ sum
Table 6
Experiment and dose: Spleen-biased reporter distribution Same 16PE formulation and IV dose; n=3 | Readout and comparator: Readout: Approximately 3 h; liver, spleen and lung signal fractions Comparator: Liver-biased library members | Result and source: Spleen dominates the displayed three-organ signal distribution; previously reviewed graph estimate approximately 90%. This is a share of reporter luminescence, not percentage of injected RNA or transfected cells. Fig. 3a,c
Table 6
Experiment and dose: Helper-lipid characterization 503O8,12 16PE, 40PE and 40TAP; n=4 | Readout and comparator: Readout: Particle size, encapsulation and zeta measurements Comparator: Three helper-lipid conditions | Result and source: SI characterization supports a physical-property comparison. It does not establish 503O8,12 lung immune-cell selectivity or the toxicity ranking reported for 514O6,10. Previously reviewed SI Fig. S1