Literature-reported formulation composition
Component: 246C10 | mol%: 26.5 | Role: Ionizable lipid
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246C10
Literature records
Search the tables and observations reproduced from the product literature guide. Rows may represent measurements, recipes or experimental context; they are not all independent experiments.
Literature-reported formulation composition
Component: DOPE | mol%: 20 | Role: Helper phospholipid
Literature-reported formulation composition
Component: Cholesterol | mol%: 52 | Role: Sterol
Literature-reported formulation composition
Component: C16-PEG2000 ceramide | mol%: 1.5 | Role: PEG lipid
Literature-reported formulation composition
Component: Reported total | mol%: 100 | Role: Source values retained
Table 2
PEG lipid: 1.0 mol% | Mean diameter: 97 ± 17 nm | PDI: 0.064 | Encapsulation efficiency: 88.3 ± 7%
Table 2
PEG lipid: 1.5 mol% | Mean diameter: 68 ± 15 nm | PDI: 0.127 | Encapsulation efficiency: 90.1 ± 5%
Table 2
PEG lipid: 3.0 mol% | Mean diameter: 58 ± 8 nm | PDI: 0.196 | Encapsulation efficiency: 80.1 ± 8%
Table 3
Condition: 3.0 mol% total PEG, no mannose | Hepatocytes: about 70% | LSECs: about 13% | Kupffer cells: about 5%
Table 3
Condition: 3.0 mol% total PEG, including 2.5 mol% mannose-PEG | Hepatocytes: 15% | LSECs: 70% | Kupffer cells: 15%
Reported observation
Literature-reported Apparent LNP pKa 6.75 246C10 mRNA-LNP Main Results: Formulation of LNPs; cited Fig.S3C
Reported observation
Literature-reported Liver-cell transfection by Cre mRNA 80% hepatocytes, 40% LSECs and 10% Kupffer cells tdTomato-positive 246C10 mCre-LNP; 0.5 mg/kg IV; n=5 · 2 days Main Results and Fig.4C
Reported observation
Literature-reported mFLuc expression Highest liver luciferase expression among 244C10, 245C10 and 246C10 formulations 0.1 mg/kg mFLuc IV in C57BL/6 mice · 3 h Main Results and Fig.3
Reported observation
Literature-reported ApoE effect in serum-free HeLa culture Approximately 400-fold increase in luciferase expression after ApoE preincubation 246C10 mFLuc-LNP with ApoE3; anti-LDLR reduced expression Main Results and Fig.2C-D
Reported observation
Mannose-directed LSEC formulation A 246C10 formulation with 3.0 mol% total PEG lipid, including 2.5 mol% mannose-PEG lipid, shifted Cre-mRNA expression toward LSECs. The remaining base lipids were 246C10/DOPE/cholesterol = 26.5/20/50.5 mol%, with 0.5 mol% non-targeted PEG lipid. 0.5 mg/kg mCre IV in LSL-tdTomato mice; n=5; tissue at 2 days · Main Results, Figs.6B and 7
Reported observation
siRNA formulation uses a different helper lipid For siRNA experiments, the article used 246C10/DSPC/cholesterol/PEG lipid = 42.5/13/41.5/3 mol% for the 3% PEG condition; 2.5 mol% mannose-PEG was incorporated for LSEC targeting. The ionizable lipid:siRNA mass ratio was 7.5:1. siFVII 0.2 mg/kg or siFVIII 0.5 mg/kg IV · Main Methods: In vivo gene silencing; Fig.8