Literature-reported formulation composition
Component: 304O13 | mol%: 50 | Role: Ionizable lipid
sales@dcchemicals.com
304O13
Literature records
Search the tables and observations reproduced from the product literature guide. Rows may represent measurements, recipes or experimental context; they are not all independent experiments.
Literature-reported formulation composition
Component: Cholesterol | mol%: 38.5 | Role: Sterol
Literature-reported formulation composition
Component: DSPC | mol%: 10.75 | Role: Helper phospholipid
Literature-reported formulation composition
Component: mPEG2000-DMG (paper MW 2660) | mol%: 0.75 | Role: PEG lipid
Literature-reported formulation composition
Component: Reported total | mol%: 100 | Role: Source values retained
Reported observation
Literature-reported Factor VII silencing Optimized 304O13 LNP EC50 0.01 mg/kg siRNA; after one 0.1 mg/kg injection, Factor VII remained suppressed for 18 days. Optimized PEG condition, anti-FVII siRNA IV · Non-targeting siRNA controls and non-optimized PEG conditions PDF p. 4, Fig. 3c-d.
Reported observation
Literature-reported Immune-cell delivery High CD45 silencing in CD11b+ monocyte/macrophage populations from peritoneal cavity and CD11c+ dendritic cells from spleen; peritoneal silencing up to approximately 90% and splenic silencing up to approximately 40% in the plotted results. 304O13 CD45-siRNA LNP, 3 days post IV injection · Non-targeting siRNA LNPs and other lipidoids PDF p. 4, Fig. 3e-f.
Reported observation
Literature-reported Biodistribution At 1 h, 304O13 Cy5.5-siRNA signal was primarily liver (42%) and spleen (24%), with 18% in kidneys; naked siRNA was 14% liver, 1% spleen and 71% kidneys. Cy5.5-siRNA, 1 mg/kg IV · Naked siRNA PDF p. 5, Fig. 4a-b.
Reported observation
Structure-function criteria Across the library, O13 tail, at least three tails and a tertiary amine were associated with potent Factor VII silencing; surface pKa of at least approximately 5.5 was also required for strong activity. These are library-level findings, not a 304O13-only causal experiment. · PDF pp. 6-7, Fig. 5 and Table 2.
Reported observation
Degradability 304O13 was hydrolyzed under acidic and basic conditions in the Methods; this demonstrates chemical degradability under the tested conditions and is not an in-vivo clearance half-life. · PDF p. 8, Lipidoid hydrolysis Methods.