Literature-reported formulation composition
Component: Imidazole cholesterol ester (ICE) | mol%: 60 | Role: Ionizable lipid
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Imidazole cholesterol ester (ICE)
Literature records
Search the tables and observations reproduced from the product literature guide. Rows may represent measurements, recipes or experimental context; they are not all independent experiments.
Literature-reported formulation composition
Component: DOPE | mol%: 35 | Role: Helper phospholipid
Literature-reported formulation composition
Component: DMG-PEG2000 | mol%: 5 | Role: PEG lipid
Literature-reported formulation composition
Component: Reported total | mol%: 100 | Role: Source values retained
Table 2
Example: 15 mg | Condition: 60/35/5 mol%; N/P 4 | Reported result: 85% encapsulation; 65 nm; PDI 0.13
Table 2
Example: 30 mg | Condition: 60/35/5 mol%; N/P 4 | Reported result: 86% encapsulation; 89 nm; PDI 0.12
Table 2
Example: 60 mg | Condition: 60/35/5 mol%; N/P 4 | Reported result: 97% encapsulation; 67 nm; PDI 0.12
Table 2
Example: 3.5 g | Condition: 60/35/5 mol%; N/P 4 | Reported result: 91% encapsulation; 50 nm; PDI 0.17
Reported observation
Literature-reported Formulation properties About 86% encapsulation; 89 nm; PDI 0.12 30 mg scale; 60/35/5 mol%; N/P 4 Paragraphs [0251]-[0252]
Reported observation
Literature-reported Scale examples 85-97% encapsulation and 50-89 nm across selected examples N/P 4; scale and cargo varied Paragraphs [0249]-[0262]
Reported observation
Literature-reported Pulmonary hCFTR protein Detected in bronchial epithelial and alveolar regions Rodent lung, 24 h after intratracheal aerosol or nebulization Paragraphs [0264]-[0281], Figs. 1-6
Reported observation
Literature-reported Expression time course Strongest staining at 24-72 h; detectable signal reported to 28 days in the rat dose study Single pulmonary exposure Paragraphs [0292]-[0299], Figs. 10-13
Reported observation
Three-component architecture The repeated examples use ICE, DOPE and DMG-PEG2000 without a separate cholesterol helper lipid. The first rapid-injection paragraph contains an inconsistent mention of cholesterol while giving only the three-part 60:35:5 ratio; the repeated pump examples resolve the operational composition. · Paragraphs [0246]-[0259]
Reported observation
Respiratory observation In the reported rat comparison, ICE-CFTR mRNA formulations did not increase respiratory rate or lung weight relative to buffer, unlike the branched-PEI comparator. Single-dose inhalation patent example; group sizes were not stated in the reviewed text. · Paragraph [0300], Fig. 14