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Instructions for Use — Lipid R6
DC60465 · Updated 18 October 2026
Description:
Lipid R6 is an AGILE-identified ionizable lipid for macrophage mRNA delivery research. Its acyclic diethylaminopropyl headgroup is linked to an asymmetric, saturated amino-amide scaffold. R6 LNPs supported higher mRNA-derived expression than H9 and MC3 LNPs in RAW 264.7 macrophages in the cited study. The reported GFP-positive population was 51.0% under the specified in vitro conditions. Its activity was cell-type dependent: H9 performed better in HeLa cells and muscle delivery. R6 is supplied as the lipid building block; formulation-specific results and composition discrepancies are detailed below.
Chemical Information
| Parameter | Value |
|---|---|
| Catalog number | DC60465 |
| Chemical Name | Lipid R6 |
| CAS No. | Not available |
| Synonyms | R6; Lipid-R6; R-6 lipid; AGILE R6; 2-((3-(diethylamino)propyl)amino)-N-undecylheptadecanamide |
| Formula | C35H73N3O |
| M.Wt | 551.99 |
| SMILES | CCCCCCCCCCCCCCCC(NCCCN(CC)CC)C(=O)NCCCCCCCCCCC |
| Purity | >95% by ELSD-HPLC |
| Storage | −20 °C; unopened, protected from light and moisture. Follow the supplied batch expiry/retest date. |
| Publication | Xu, Y.; Ma, S.; Cui, H.; et al. AGILE platform: a deep learning powered approach to accelerate LNP development for mRNA delivery. Nature Communications 2024, 15, 6305. DOI: 10.1038/s41467-024-50619-z |
Handling:
Store the unopened product at −20 °C, protected from light and moisture, with the vial tightly sealed. The standard storage period is 1 year; follow the expiry or retest date on the supplied batch documentation. Allow the closed vial to reach room temperature before opening to avoid condensation.
We ship the pure lipid with ice packs, without ethanol. Prepare an ethanol stock solution when required. Gentle warming and brief sonication may assist dissolution; confirm complete dissolution at the intended concentration.
Prepare and use solutions on the same day wherever possible. If advance preparation is required, use a validated storage procedure and tightly sealed aliquots. A general solution shelf life is not assigned.
For small amounts of oily or viscous lipid, dissolve the entire pack and take volumetric aliquots. For example, dissolve 25 mg and adjust to a final volume of 1 mL to obtain 25 mg/mL; a 100 µL aliquot contains 2.5 mg.
R6-specific long-term stock-solution stability is not established. The cited optimized composition is inconsistent between main Methods and Supplementary Table 4.
Formulation & Performance:
R6 was evaluated for RAW 264.7 macrophage mRNA delivery. The reported GFP-positive population is 51.0% in Fig. 4g at 500 ng GFP mRNA per well and a 48 h endpoint. Separate muscle delivery and OVA-vaccine experiments do not show a general in vivo advantage.
References:
Xu, Y.; Ma, S.; Cui, H.; et al. AGILE platform: a deep learning powered approach to accelerate LNP development for mRNA delivery. Nature Communications 2024, 15, 6305. DOI: 10.1038/s41467-024-50619-z.
Supplementary Information, pp. 1–57 · Source Data workbook.
Study content is paraphrased and calculations are identified; source article licensed under CC BY 4.0.