Lipid R6
Datasheet

Product identity and specifications

Product description

Product overview Description & Application Lipid R6 is an AGILE-identified ionizable lipid for macrophage mRNA delivery research. Its acyclic diethylaminopropyl headgroup is linked to an asymmetric, saturated amino-amide scaffold. R6 LNPs supported higher mRNA-derived expression than H9 and MC3 LNPs in RAW 264.7 macrophages in the cited study. The reported GFP-positive population was 51.0% under the specified in vitro conditions. Its activity was cell-type dependent: H9 performed better in HeLa cells and muscle delivery. R6 is supplied as the lipid building block; formulation-specific results and composition discrepancies are detailed below. RNA Delivery Cationic and ionizable lipids Featured lipid products

Handling guidance

Handling guidance Shipping, storage and sampling How is the lipid shipped, and how should I store it? We generally ship the neat (undissolved) lipid compound rather than an ethanol solution. The neat lipid is stable during transport with ice packs, and shipments originate from Shanghai. Pure form: store at −20 °C for up to 1 year. Solution shelf life is not established for this product; use a validated solvent and aliquoting procedure. Keep sealed and protected from light. Avoid repeated thaw cycles for best results. Pure lipid or solution — which should I choose? Pure lipid is generally more stable during shipping and storage, so we usually recommend the pure form. A solution in ethanol or chloroform can be supplied when required by your workflow. Tell us the intended application, solvent and target concentration so the most suitable format can be confirmed. How should I measure a small amount of oily or viscous lipid? Ionizable lipids are often oily liquids or viscous semisolids, so material can be lost during repeated weighing or transfer. For a small pack, dissolve the entire quantity and aliquot it volumetrically. For example, add 1 mL ethanol to 25 mg lipid to prepare a 25 mg/mL stock, then use a pipette to withdraw the required amount. For quantities above 100 mg, direct weighing with an analytical balance may be more practical. Always confirm solvent compatibility and solubility first. Can you support formulation optimization and scale-up? Yes. Share the RNA cargo, target tissue, administration route, formulation method and assay plan. Our team can discuss starting molar ratios, N/P ratio and scale-up requirements for research workflows. What is specific to Lipid R6? R6 is supplied as an unformulated lipid building block. The reported macrophage data depend on the complete LNP formulation. Its saturated tail scaffold has no ester or disulfide linkage; do not assume the degradability profile of H9. Product-specific long-term stock-solution stability is not established.

Formulation and performance

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Search the retained literature tables and observations.

Ordering

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References

• DOI: 10.1038/s41467-024-50619-z