Literature-reported formulation composition
Component: DODAP | mol%: 50 | Role: Ionizable lipid
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DODAP
Literature records
Search the tables and observations reproduced from the product literature guide. Rows may represent measurements, recipes or experimental context; they are not all independent experiments.
Literature-reported formulation composition
Component: Cholesterol | mol%: 39 | Role: Sterol
Literature-reported formulation composition
Component: DOPE | mol%: 11 | Role: Helper phospholipid
Literature-reported formulation composition
Component: Reported total | mol%: 100 | Role: Source values retained
Table 3
Measurement: Diameter (nm) | Initial core: 148 ± 2 | Three-layer HA-LNP: 174 ± 1
Table 3
Measurement: PDI | Initial core: 0.20 ± 0.01 | Three-layer HA-LNP: 0.18 ± 0.01
Table 3
Measurement: RNA assay (%) | Initial core: 86 ± 1 (core EE) | Three-layer HA-LNP: 86 ± 1 (layer RNA; indirect)
Reported observation
Literature-reported Three-layer particle diameter 174 ± 1 nm Mean ± SD; DLS Z-average Main Results 3.1; Fig.1
Reported observation
Literature-reported Three-layer PDI 0.18 ± 0.01 Mean ± SD; DLS Main Results 3.1; Fig.1
Reported observation
Literature-reported Core siRNA encapsulation 86 ± 1% SYBR Gold; disrupted versus intact cores Main Results 3.1; Fig.1
Reported observation
Literature-reported Layer-associated siRNA (indirect) 86 ± 1% Indirect SYBR Gold accessibility estimate; not a direct recovery assay Main Results 3.1; Fig.1
Reported observation
Literature-reported Residual luciferase activity 49 ± 1% of untreated cells Three-layer HA-LNP; siLuc 180 nM; GL261-Luc cells · 48 h · Untreated cells; scrambled-siRNA particle control Main Results 3.1; Fig.1
Reported observation
Coating composition is additional to the lipid core The 50/39/11 mol% table covers lipid core components only. To obtain the measured three-layer particles, 10 microlitres of 20 micromolar siRNA, 1 microlitre of 2 mg/mL poly-L-arginine and 5 microlitres of 5 mg/mL hyaluronic acid were added sequentially to 14.1 microlitres core suspension. Ten minutes at room temperature after each layer · Methods 2.2
Reported observation
In vitro tolerability scope No significant loss of cell viability was reported for the three-layer particles at 180 nM siRNA in the 48 h GL261-Luc assay. This is not systemic or long-term safety evidence. WST-8 viability alongside luciferase assay · Methods 2.5; Results 3.1; Fig.1D
Reported observation
DODAP requires a different first-layer amount Using 7 microlitres of 20 micromolar siRNA caused aggregation above 1 micrometre; increasing this to 10 microlitres enabled charge inversion and stable assembly. DODMA and MC3 used 7 microlitres. 14.1 microlitres core suspension; 10 mM acetate pH 4 · Discussion; Methods 2.2